PIH1D1 Polyclonal antibody, PBS Only

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  WB analysis using 19427-1-AP (same clone as 19427-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 19427-1-AP (same clone as 19427-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse eye using 19427-1-AP (same clone as 19427-1-PBS)

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  WB analysis of human uterus using 19427-1-AP (same clone as 19427-1-PBS)

  human uterus tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 19427-1-AP (same clone as 19427-1-PBS)

  IP result of anti-PIH1D1 (IP:19427-1-AP, 4ug; Detection:19427-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 19427-1-AP (same clone as 19427-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 19427-1-AP (same clone as 19427-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 19427-1-AP (same clone as 19427-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 19427-1-AP (same clone as 19427-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 19427-1-AP (same clone as 19427-1-PBS)

  Immunofluorescent analysis of Hela cells, using PIH1D1 antibody 19427-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

PIH1D1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 19427-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

19427-1-PBS targets PIH1D1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13759

  Product name: Recombinant human PIH1D1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-290 aa of BC001108

  Sequence: MANPKLLGLELSEAEAIGADSARFEELLLQASKELQQAQTTRPESTQIQPQPGFCIKTNSSEGKVFINICHSPSIPPPADVTEEELLQMLEEDQAGFRIPMSLGEPHAELDAKGQGCTAYDVAVNSDFYRRMQNSDFLRELVITIAREGLEDKYNLQLNPEWRMMKNRPFMGSISQQNIRSEQRPRIQELGDLYTPAPGRAESGPEKPHLNLWLEAPDLLLAEIDLPKLDGALGLSLEIGENRLVMGGPQQLYHLDAYIPLQINSHESKAAFHRKRKQLMVAMPLLLVPS

• Full Name: PIH1 domain containing 1
• Calculated Molecular Weight: 290 aa, 32 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC001108
• Gene Symbol: PIH1D1
• Gene ID (NCBI): 55011
• RRID: AB_10640423
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NWS0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PIH1D1 (PIH1 domain-containing protein 1; also known as PIH1 or NOP17), is a ortholog of the yeast nucleolar protein NOP17. PIH1D1 is a conserved subunit of the R2TP complex participating in the assembly of snoRNP and RNA polymerase II complexes. PIH1D1 may also function as a novel modulator of apoptosis pathway.