PIH1D1 Polyclonal antibody

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  WB analysis using 19427-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human uterus using 19427-1-AP

  human uterus tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 19427-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse eye using 19427-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19427-1-AP

  IP result of anti-PIH1D1 (IP:19427-1-AP, 4ug; Detection:19427-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of human lung using 19427-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 19427-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 19427-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 19427-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 19427-1-AP

  Immunofluorescent analysis of Hela cells, using PIH1D1 antibody 19427-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

PIH1D1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 19427-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

PIH1 domain-containing protein 1, PIH1 domain containing 1, Nucleolar protein 17 homolog, NOP17

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Tested Applications

• Positive WB detected in: A431 cells, human testis tissue, human uterus tissue, mouse eye tissue, HeLa cells, mouse ovary tissue, mouse testis tissue, rat testis tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

19427-1-AP targets PIH1D1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13759

  Product name: Recombinant human PIH1D1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-290 aa of BC001108

  Sequence: MANPKLLGLELSEAEAIGADSARFEELLLQASKELQQAQTTRPESTQIQPQPGFCIKTNSSEGKVFINICHSPSIPPPADVTEEELLQMLEEDQAGFRIPMSLGEPHAELDAKGQGCTAYDVAVNSDFYRRMQNSDFLRELVITIAREGLEDKYNLQLNPEWRMMKNRPFMGSISQQNIRSEQRPRIQELGDLYTPAPGRAESGPEKPHLNLWLEAPDLLLAEIDLPKLDGALGLSLEIGENRLVMGGPQQLYHLDAYIPLQINSHESKAAFHRKRKQLMVAMPLLLVPS

• Full Name: PIH1 domain containing 1
• Calculated Molecular Weight: 290 aa, 32 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC001108
• Gene Symbol: PIH1D1
• Gene ID (NCBI): 55011
• RRID: AB_10640423
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NWS0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIH1D1 (PIH1 domain-containing protein 1; also known as PIH1 or NOP17), is a ortholog of the yeast nucleolar protein NOP17. PIH1D1 is a conserved subunit of the R2TP complex participating in the assembly of snoRNP and RNA polymerase II complexes. PIH1D1 may also function as a novel modulator of apoptosis pathway.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IHC	Nat Commun; The HSP90/R2TP assembly chaperone promotes cell proliferation in the intestinal epithelium.; Authors - Chloé Maurizy
rat	WB	Front Cell Dev Biol; PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia; Authors - Tingting Zhang
human		RNA; Mechanism of the AAA+ ATPases pontin and reptin in the biogenesis of H/ACA RNPs.; Authors - Machado-Pinilla Rosario R
human	WB	Cell Chem Biol; RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.; Authors - Paul Yenerall
mouse	WB	Chromosoma; The PRDM9 KRAB domain is required for meiosis and involved in protein interactions.; Authors - Yukiko Imai
mouse	WB	J Biol Chem; Nrf1-mediated transcriptional regulation of the proteasome requires a functional TIP60 complex.; Authors - Janakiram R Vangala