PISD Monoclonal antibody

PISD Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3B6C1

Cat no : 68484-1-Ig

Synonyms

DJ858B16, dJ858B16.2, DKFZp566G2246, PISD, PSDC, PSSC

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Validation Data Gallery

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  WB analysis using 68484-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68484-1-Ig (PISD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68484-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68484-1-Ig (PISD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68484-1-Ig targets PISD in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PISD fusion protein Ag9499
• Full Name: phosphatidylserine decarboxylase
• Calculated Molecular Weight: 375aa,43 kDa; 408aa,46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC001482
• Gene Symbol: PISD
• Gene ID (NCBI): 23761
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PISD(Phosphatidylserine decarboxylase proenzyme) belongs to the phosphatidylserine decarboxylase family.It contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits.It has 2 isoforms produced by alternative splicing.