PITPNM2/NIR3 Polyclonal antibody

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• PITPNM2/NIR3 Polyclonal antibody (21169-1-AP)

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  IHC staining of human placenta using 21169-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21169-1-AP (PITPNM2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse placenta using 21169-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 21169-1-AP (PITPNM2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PITPNM2/NIR3 Polyclonal Antibody for IHC, ELISA

Cat No. 21169-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

PITPNM2, KIAA1457, Membrane-associated phosphatidylinositol transfer protein 2, NIR3, NIR-3

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Tested Applications

• Positive IHC detected in: human placenta tissue, mouse placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21169-1-AP targets PITPNM2/NIR3 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15385

  Product name: Recombinant human PITPNM2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 233-328 aa of BC141813

  Sequence: EWYGLSMENIRELEKEAQLMLSRKMAQFNEDGEEATELVKHEAVSDQTSGEPPEPSSSNGEPLVGRGLKKQWSTSSKSSRSSKRGASPSRHSISEW

• Full Name: phosphatidylinositol transfer protein, membrane-associated 2
• Calculated Molecular Weight: 1349 aa, 149 kDa
• GenBank Accession Number: BC141813
• Gene Symbol: PITPNM2
• Gene ID (NCBI): 57605
• RRID: AB_3742019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BZ72
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PITPNM2, also known as NIR3, belongs to a family of membrane-associated phosphatidylinositol transfer domain-containing proteins that share homology with the Drosophila retinal degeneration B (rdgB) protein(PMID: 15627748). PITPNM2/NIR3 promotes both TCRβ selection and positive selection in thymocytes. PITPNM2/NIR3 is required for PIP2 replenishment and TCR-induced calcium signaling in response to weak TCR stimulation(PMID: 36581712).