Tested Applications
| Positive WB detected in | rat brain tissue, HEK-293 cells, mouse brain tissue, rat cerebellum tissue, mouse cerebellum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IF | See 1 publications below |
Product Information
67201-1-Ig targets PITX2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27924 Product name: Recombinant human PITX2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-286 aa of BC013998 Sequence: MNCMKGPLHLEHRAAGTKLSAVSSSSCHHPQPLAMASVLAPGQPRSLDSSKHRLEVHTISDTSSPEAAEKDKSQQGKNEDVGAEDPSKKKRQRRQRTHFTSQQLQELEATFQRNRYPDMSTREEIAVWTNLTEARVRVWFKNRRAKWRKRERNQQAELCKNGFGPQFNGLMQPYDDMYPGYSYNNWAAKGLTSASLSTKSFPFFNSMNVNPLSSQSMFSPPNSISSMSMSSSMVPSAVTGVPGSSLNSLNNLNNLSSPSLNSAVPTPACPYAPPTPPYVYRDTCNS Predict reactive species |
| Full Name | paired-like homeodomain 2 |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC013998 |
| Gene Symbol | PITX2 |
| Gene ID (NCBI) | 5308 |
| RRID | AB_2882494 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q99697 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PITX2 belongs to the bicoid class of homeodomain transcription factors, which play essential roles in embryonic development. Various PITX2 isoforms are involved in a wide variety of developmental programs, including left-right signaling. It controls cell proliferation in a tissue-specific manner and is involved in morphogenesis. During embryonic development, PITX2 is involved in the expansion of muscle progenitors. In addition, it may play a role in the proper localization of asymmetric organs such as the heart and stomach. PITX2 can be bound by some other proteins, and are part of a much larger atrial transcriptional complex. This antibody specifically recognizes isoform PTX2C.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PITX2 antibody 67201-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oral Dis Enamel defects of Axenfeld-Rieger syndrome and the role of PITX2 in its pathogenesis.
| ||
Aging (Albany NY) D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism
| ||
Genomics Proteomics Bioinformatics Single-cell Atlas of Developing Mouse Palates Reveals Cellular and Molecular Transitions in Periderm Cell Fate |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kenzo (Verified Customer) (03-28-2024) | Worked well for immunofluorescence in mouse heart tissues.
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