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  • KD/KO Validated

PKM2-specific Monoclonal antibody

PKM2-specific Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ICC, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1C11C7

Cat no : 60268-1-Ig

Synonyms

CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM2, PKM2 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK



Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue, MCF-7 cells, C6 cells, NIH/3T3 cells, rat brain tissue, HepG2 cells, HSC-T6 cells, ROS1728 cells, 4T1 cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:30000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

60268-1-Ig targets PKM2-specific in WB, IP, IHC, IF, ICC, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name PKM pyruvate kinase, muscle
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberNM_002654
Gene symbol PKM
Gene ID (NCBI) 5315
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms,the activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.

Protocols

Product Specific Protocols
WB protocol for PKM2-specific antibody 60268-1-IgDownload protocol
IHC protocol for PKM2-specific antibody 60268-1-IgDownload protocol
IF protocol for PKM2-specific antibody 60268-1-IgDownload protocol
IP protocol for PKM2-specific antibody 60268-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Mol Cell

The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.

Authors - Youming Zhu
humanWB

Autophagy

SQSTM1-dependent autophagic degradation of PKM2 inhibits the production of mature IL1B/IL-1β and contributes to LIPUS-mediated anti-inflammatory effect.

Authors - Bin Zhang
humanWB,IHC,IF

Mol Ther Nucleic Acids

Endoplasmic reticulum stress promotes sorafenib resistance via miR-188-5p/hnRNPA2B1-mediated upregulation of PKM2 in hepatocellular carcinoma

Authors - Bei Zhou
  • KD Validated
mouseWB

J Am Heart Assoc

Pyruvate Kinase M2 Protects Heart from Pressure Overload-Induced Heart Failure by Phosphorylating RAC1.

Authors - Le Ni
humanIF

Front Pharmacol

Qian Yang Yu Yin Granule Improves Renal Injury of Hypertension by Regulating Metabolic Reprogramming Mediated by HIF-1α/PKM2 Positive Feedback Loop.

Authors - Lichao Qian
ratWB

Front Pharmacol

Danqi Pill Protects Against Heart Failure Post-Acute Myocardial Infarction via HIF-1α/PGC-1α Mediated Glucose Metabolism Pathway.

Authors - Qian Zhang