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PKM2-specific Monoclonal antibody
PKM2-specific Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 60268-1-Ig
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, human brain tissue, MCF-7 cells, C6 cells, NIH/3T3 cells, rat brain tissue, HepG2 cells, HSC-T6 cells, ROS1728 cells, 4T1 cells, K-562 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:30000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:1000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
60268-1-Ig targets PKM2-specific in WB, IP, IHC, IF, ICC, FC, CoIP applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Full Name||PKM pyruvate kinase, muscle|
|Calculated molecular weight||58 kDa|
|Observed molecular weight||58 kDa|
|GenBank accession number||NM_002654|
|Gene ID (NCBI)||5315|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms,the activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.
|Product Specific Protocols|
|WB protocol for PKM2-specific antibody 60268-1-Ig||Download protocol|
|IHC protocol for PKM2-specific antibody 60268-1-Ig||Download protocol|
|IF protocol for PKM2-specific antibody 60268-1-Ig||Download protocol|
|IP protocol for PKM2-specific antibody 60268-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
J Exp Clin Cancer Res
PKM2 is the target of proanthocyanidin B2 during the inhibition of hepatocellular carcinoma.
J Exp Clin Cancer Res
miR-122 inhibits cancer cell malignancy by targeting PKM2 in gallbladder carcinoma.
Acta Physiol (Oxf)
Supra-coronary aortic banding improves right ventricular function in experimental pulmonary arterial hypertension in rats by increasing systolic right coronary artery perfusion.
Acta Pharm Sin B
Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.
Mol Biol Rep
Development of a one-plasmid system to replace the endogenous protein with point mutation for post-translational modification studies.