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PKM2-specific Monoclonal antibody
PKM2-specific Monoclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat no : 60268-1-Ig
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, human brain tissue, MCF-7 cells, C6 cells, NIH/3T3 cells, rat brain tissue, HepG2 cells, HSC-T6 cells, ROS1728 cells, 4T1 cells, K-562 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:30000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:1000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
60268-1-Ig targets PKM2-specific in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Full Name||PKM pyruvate kinase, muscle|
|Calculated molecular weight||58 kDa|
|Observed molecular weight||58 kDa|
|GenBank accession number||NM_002654|
|Gene ID (NCBI)||5315|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms,the activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.
|Product Specific Protocols|
|WB protocol for PKM2-specific antibody 60268-1-Ig||Download protocol|
|IHC protocol for PKM2-specific antibody 60268-1-Ig||Download protocol|
|IF protocol for PKM2-specific antibody 60268-1-Ig||Download protocol|
|IP protocol for PKM2-specific antibody 60268-1-Ig||Download protocol|
|FC protocol for PKM2-specific antibody 60268-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
J Exp Clin Cancer Res
PKM2 is the target of proanthocyanidin B2 during the inhibition of hepatocellular carcinoma.
J Exp Clin Cancer Res
miR-122 inhibits cancer cell malignancy by targeting PKM2 in gallbladder carcinoma.
Lipids Health Dis
Dietary fish oil supplement induces age-specific contractile and proteomic responses in muscles of male rats.
Drug Des Devel Ther
Dihydroartemisinin Inhibits the Proliferation of Leukemia Cells K562 by Suppressing PKM2 and GLUT1 Mediated Aerobic Glycolysis.
Shikonin differentially regulates glucose metabolism via PKM2 and HIF1α to overcome apoptosis in a refractory HCC cell line.