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PKM2-specific Monoclonal antibody

PKM2-specific Monoclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

CloneNo.

1C11C7

Cat no : 60268-1-Ig

Synonyms

CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM2, PKM2 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK



Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue, MCF-7 cells, C6 cells, NIH/3T3 cells, rat brain tissue, HepG2 cells, HSC-T6 cells, ROS1728 cells, 4T1 cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:30000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

60268-1-Ig targets PKM2-specific in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name PKM pyruvate kinase, muscle
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberNM_002654
Gene symbol PKM
Gene ID (NCBI) 5315
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms,the activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.

Protocols

Product Specific Protocols
WB protocol for PKM2-specific antibody 60268-1-IgDownload protocol
IHC protocol for PKM2-specific antibody 60268-1-IgDownload protocol
IF protocol for PKM2-specific antibody 60268-1-IgDownload protocol
IP protocol for PKM2-specific antibody 60268-1-IgDownload protocol
FC protocol for PKM2-specific antibody 60268-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Exp Clin Cancer Res

PKM2 is the target of proanthocyanidin B2 during the inhibition of hepatocellular carcinoma.

Authors - Jiao Feng
humanWB,IF

J Exp Clin Cancer Res

PMID: 32000827

Authors - Jiao Feng
humanIHC

Tumour Biol

miR-122 inhibits cancer cell malignancy by targeting PKM2 in gallbladder carcinoma.

Authors - Wei Lu
ratWB

Lipids Health Dis

Dietary fish oil supplement induces age-specific contractile and proteomic responses in muscles of male rats.

Authors - David W Russ
humanWB,IF

Drug Des Devel Ther

Dihydroartemisinin Inhibits the Proliferation of Leukemia Cells K562 by Suppressing PKM2 and GLUT1 Mediated Aerobic Glycolysis.

Authors - Peng Gao
humanWB,IF

Life Sci

Shikonin differentially regulates glucose metabolism via PKM2 and HIF1α to overcome apoptosis in a refractory HCC cell line.

Authors - Wei Yang