Tested Applications
| Positive WB detected in | A2780 cells, U-251 cells, U-87 MG cells |
| Positive IF/ICC detected in | U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10286-1-AP targets uPAR/CD87 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0187 Product name: Recombinant human uPAR, PLAUR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-136 aa of BC002788 Sequence: MGHPPLLPLLLLLHTCVPASWGLRCMQCKTNGDCRVEECALGQDLCRTTIVRLWEEGEELELVEKSCTHSEKTNRTLSYRTGLKITSLTEVVCGLDLCNQGNSGRAVTYSRSRYLECISCGSSDMSCERGRHQSLQ Predict reactive species |
| Full Name | PLAUR |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 40-50 kDa |
| GenBank Accession Number | BC002788 |
| Gene Symbol | uPAR |
| Gene ID (NCBI) | 5329 |
| RRID | AB_10667460 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q03405 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
uPAR is a 45-65 kDa, highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodelling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for uPAR/CD87 antibody 10286-1-AP | Download protocol |
| WB protocol for uPAR/CD87 antibody 10286-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The uPAR/CD87 Polyclonal antibody (Cat# 10286-1-AP) has 38 citations. Journals include Cancer Research, Nature Communications, Molecular Cell, Nucleic Acids Research, Cell Reports Medicine, FASEB Journal, Journal of Virology, Biophysical Journal, and Frontiers in Oncology, among others. Research fields span oncology (breast, thyroid, bladder, renal, colorectal, glioma, cholangiocarcinoma), podocyte/kidney injury, bone loss/osteoclastogenesis, inflammation, vasculitis, fibrosis, viral infection, and uPAR signaling/biomarker studies.
| Species | Application | Title |
|---|---|---|
Cancer Res Bcl-xL enforces a slow-cycling state necessary for survival in the nutrient-deprived microenvironment of pancreatic cancer. | ||
Clin Mol Hepatol Radiogenomics of intrahepatic cholangiocarcinoma predicts immunochemotherapy response and identifies therapeutic target | ||
Nat Commun Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling. | ||
J Adv Res Discovery of FBP1 as novel therapeutic target and asiatic acid-hydrogen sulfide donors accelerate diabetic wound healing. | ||
Mol Cell A transient transcriptional activation governs unpolarized-to-polarized morphogenesis during embryo implantation | ||
Nucleic Acids Res Regulation of u-PAR gene expression by H2A.Z is modulated by the MEK-ERK/AP-1 pathway. |
Reviews
What customers say
The single review for this product is a 5-star rating from an immunofluorescence experiment on mouse skin tissue. The reviewer used a 1:50 dilution and reported successful IF results. Overall, the feedback is positive, confirming the antibody works well for immunofluorescence applications in mouse tissue.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ruchi (Verified Customer) (07-26-2022) | IF on mouse skin
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