uPAR Polyclonal antibody

uPAR Polyclonal Antibody for FC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10286-1-AP

Synonyms

CD87, MO3, PLAUR, U PAR, UPAR, URKR



Tested Applications

Positive WB detected inU-937 cells, A2780 cells, RAW 264.7 cells
Positive IP detected inRAW 264.7 cells
Positive FC detected inU-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 10286-1-AP is uPAR Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen uPAR fusion protein Ag0187
Full Name PLAUR
Calculated molecular weight 37 kDa
Observed molecular weight 35-70 kDa
GenBank accession numberBC002788
Gene symbol PLAUR
Gene ID (NCBI) 5329
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

uPAR is a 45-65 kDa, highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodelling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)

Protocols

Product Specific Protocols
WB protocol for uPAR antibody 10286-1-APDownload protocol
IP protocol for uPAR antibody 10286-1-APDownload protocol
FC protocol for uPAR antibody 10286-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.

Authors - Min Liu
mouseIHC

Cancer Res

Bcl-xL enforces a slow-cycling state necessary for survival in the nutrient-deprived microenvironment of pancreatic cancer.

Authors - Yogev Sela
humanWB

Nucleic Acids Res

Regulation of u-PAR gene expression by H2A.Z is modulated by the MEK-ERK/AP-1 pathway.

Authors - Santosh Chauhan
human,mouseWB,IHC

FASEB J

CLEC14A protects against podocyte injury in mice with adriamycin nephropathy.

Authors - Zeyu Su
humanIHC

J Inflamm Res

PLAUR as a Potential Biomarker Associated with Immune Infiltration in Bladder Urothelial Carcinoma.

Authors - Mulin Liu
humanWB

Front Oncol

Forkhead Box Q1 Is Critical to Angiogenesis and Macrophage Recruitment of Colorectal Cancer.

Authors - Hui Tang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ruchi (Verified Customer) (07-26-2022)

IF on mouse skin

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Mouse skin
uPAR Antibody Immunofluorescence validation (1:50 dilution) in Mouse skin (Cat no:10286-1-AP)