|Positive WB detected in||RAW 264.7 cells, A2780 cells, U-251 cells, U-937 cells|
|Positive IP detected in||RAW 264.7 cells|
|Positive FC detected in||U-937 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
10286-1-AP targets uPAR in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||uPAR fusion protein Ag0187|
|Calculated molecular weight||37 kDa|
|Observed molecular weight||35-70 kDa|
|GenBank accession number||BC002788|
|Gene ID (NCBI)||5329|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
uPAR is a 45-65 kDa, highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodelling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ruchi (Verified Customer) (07-26-2022)
IF on mouse skin