Phospholipase C Beta 1 Polyclonal antibody
Phospholipase C Beta 1 Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
WB, IHC,ELISA
Conjugate
Unconjugated
Cat no : 26551-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IHC detected in | human gliomas tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Product Information
The immunogen of 26551-1-AP is Phospholipase C Beta 1 Fusion Protein expressed in E. coli.
Tested Reactivity | human, rat, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Phospholipase C Beta 1 fusion protein Ag24750 |
Full Name | phospholipase C, beta 1 (phosphoinositide-specific) |
Calculated molecular weight | 1216 aa, 139 kDa |
Observed molecular weight | 139 kDa |
GenBank accession number | BC069420 |
Gene symbol | PLCB1 |
Gene ID (NCBI) | 23236 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PLCB1 catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. PLCB1 is activated by two G-protein alpha subunits, alpha-q and alpha-11.
Protocols
Product Specific Protocols | |
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WB protocol for Phospholipase C Beta 1 antibody 26551-1-AP | Download protocol |
IHC protocol for Phospholipase C Beta 1 antibody 26551-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |