Validation Data Gallery
|Positive WB detected in
|mouse brain tissue, rat brain tissue
|Positive IHC detected in
|human gliomas tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:500-1:2000
|IHC : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
The immunogen of 26551-1-AP is Phospholipase C Beta 1 Fusion Protein expressed in E. coli.
|human, rat, mouse
|Host / Isotype
|Rabbit / IgG
|Phospholipase C Beta 1 fusion protein Ag24750
|phospholipase C, beta 1 (phosphoinositide-specific)
|Calculated molecular weight
|1216 aa, 139 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
PLCB1 catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. PLCB1 is activated by two G-protein alpha subunits, alpha-q and alpha-11.
PLCB1 Enhances Cell Migration and Invasion in Gastric Cancer Via Regulating Actin Cytoskeletal Remodeling and Epithelial-Mesenchymal Transition
Front Endocrinol (Lausanne)
Estrogen receptor subtype mediated anti-inflammation and vasorelaxation via genomic and nongenomic actions in septic mice
Nat Chem Biol
ENO1 promotes liver carcinogenesis through YAP1-dependent arachidonic acid metabolism