Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17421-1-AP targets PLCL2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11443 Product name: Recombinant human PLCL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 653-1001 aa of BC036392 Sequence: VDPYVYVEIHGIPADCAEQRTKTVHQNGDAHIFDESFEFQINLPELAMVRFVVLDDDYIGDEFIGQYTIPFECLQTGYRHVPLQSLTGEVLAHASLFVHVAITNRRGGGKPRKRGLSVRKGKKSREYASLRTLWIKTVDEVFKNAQPPIRDATDLRENMQNAVVSFKELCGLSSVANLMQCMLAVSPRFLGPDNTPLVVLNLSEQYPTMELQGIVPEVLKKIVTTYDMMIQSLKALIENADAVYEKIVHCQKAAMEFHEHLHSIGTKEGLKERKLQKAVESFTWNITILKGQADLLKYAKNETLENLKQIHFAAVSCGLNKPGTENADVQKPRRSLEVIPEKANDETGE Predict reactive species |
| Full Name | phospholipase C-like 2 |
| Calculated Molecular Weight | 126 kDa |
| Observed Molecular Weight | 126 kDa |
| GenBank Accession Number | BC036392 |
| Gene Symbol | PLCL2 |
| Gene ID (NCBI) | 23228 |
| RRID | AB_2163732 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UPR0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PLCL2, a novel phospholipase C-like protein, is expressed in lymphocytes and platelets. The expression of PLCL2 is associated with the proliferation of mature B cells in the immune system. PLCL2 is a new susceptibility loci for myocardial infarction. It has been shown that PLCL2 plays a key role in the pathogenesis of atherosclerosis and systemic sclerosisit. There are three isoforms of PLCL2 protein and 17421-1-AP antibody detects the 126 kDa band in SDS-PAGE. (PMID: 24916648, 25880423)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PLCL2 antibody 17421-1-AP | Download protocol |
| WB protocol for PLCL2 antibody 17421-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PLCL2 Polyclonal antibody (Cat# 17421-1-AP) has 2 citations published in Proceedings of the National Academy of Sciences (PNAS) and Frontiers in Physiology. The associated research fields include progesterone receptor isoform B–mediated regulation of uterine contractility via the Oxtr-PLCβ2-TrPC3 pathway, and TGR5 bile acid receptor–driven GLP-1/PYY release modulated by the Epac/PLC-ε pathway. Both studies employed Western blot in mouse models.
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Progesterone receptor isoform B regulates the Oxtr-Plcl2-Trpc3 pathway to suppress uterine contractility. | ||
Front Physiol Release of GLP-1 and PYY in response to the activation of G protein-coupled bile acid receptor TGR5 is mediated by Epac/PLC-ε pathway and modulated by endogenous H2S. |
