PLEK Monoclonal antibody

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• KD/KO Validated

PLEK Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1D5C2

Cat no : 66431-1-Ig

Synonyms

P47, Platelet 47 kDa protein, pleckstrin, PLEK

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Validation Data Gallery

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  WB analysis of U-937 using 66431-1-Ig

  WB result of PLEK antibody (66431-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLEK transfected U-937 cells.

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  WB analysis of HL-60 using 66431-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66431-1-Ig (PLEK Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 66431-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66431-1-Ig (PLEK Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 66431-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 66431-1-Ig (PLEK Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig spleen using 66431-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66431-1-Ig (PLEK Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66431-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66431-1-Ig (PLEK Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66431-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66431-1-Ig (PLEK Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 66431-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using PLEK antibody (66431-1-Ig, Clone: 1D5C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse spleen using 66431-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using PLEK antibody (66431-1-Ig, Clone: 1D5C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HL-60 cells, pig spleen tissue, THP-1 cells, U-937 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

66431-1-Ig targets PLEK in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig, mouse samples.

• Tested Reactivity: human, pig, mouse
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PLEK fusion protein Ag3187
• Full Name: pleckstrin
• Calculated Molecular Weight: 350 aa, 40 kDa
• Observed Molecular Weight: 40-47 kDa
• GenBank Accession Number: BC018549
• Gene Symbol: PLEK
• Gene ID (NCBI): 5341
• RRID: AB_2881802
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLEK (pleckstrin; also known as p47) was originally identified as the major PKC substrate in platelets and later is found to be expressed in all cells of the hemopoietic system. Through two pleckstrin homology (PH) domains at its N and C termini, PLEK may interact with various protein and/or lipid ligands and serve as an intracellular adaptor/targeting protein. Predominantly cytosolic in unstimulated cells, PLEK would undergo transient redistribution to phagosomal membrane in response to stimuli. PLEK has been found to be hyperphosphorylated in diabetic mononuclear phagocytes and promote proinflammatory cytokine secretion in diabetes. (10477609, 17579087)

Publications

Species	Application	Title
human	IHC,IF	Aging (Albany NY); Comprehensive analysis identifies crucial genes associated with immune cells mediating progression of carotid atherosclerotic plaque; Authors - Zhen Li