CoraLite® Plus 488-conjugated PLEK Monoclonal antibody

• Featured Product
• KD/KO Validated

PLEK Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D5C2

Cat no : CL488-66431

Synonyms

FLJ27168, P47, Platelet 47 kDa protein, pleckstrin, PLEK

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Validation Data Gallery

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  WB analysis using CL488-66431

  HL-60 cells were subjected to SDS PAGE followed by western blot with CL488-66431 (PLEK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HL-60 cells, THP-1 cells, U-937 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66431 targets PLEK in WB applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PLEK fusion protein Ag3187
• Full Name: pleckstrin
• Calculated Molecular Weight: 350 aa, 40 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC018549
• Gene Symbol: PLEK
• Gene ID (NCBI): 5341
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLEK (pleckstrin; also known as p47) was originally identified as the major PKC substrate in platelets and later is found to be expressed in all cells of the hemopoietic system. Through two pleckstrin homology (PH) domains at its N and C termini, PLEK may interact with various protein and/or lipid ligands and serve as an intracellular adaptor/targeting protein. Predominantly cytosolic in unstimulated cells, PLEK would undergo transient redistribution to phagosomal membrane in response to stimuli. PLEK has been found to be hyperphosphorylated in diabetic mononuclear phagocytes and promote proinflammatory cytokine secretion in diabetes. (10477609, 17579087)