Perilipin 1 Polyclonal antibody

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  WB analysis using 27716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27716-1-AP (Perilipin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27716-1-AP (PLIN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brown adipose using 27716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 27716-1-AP (Perilipin 1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brown adipose using 27716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 27716-1-AP (Perilipin 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brown adipose using 27716-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brown adipose tissue using PLIN1 antibody (27716-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brown adipose using 27716-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brown adipose tissue using PLIN1 antibody (27716-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Perilipin 1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 27716-1-AP

Publications(9)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF-P, ELISA

Synonyms

PLIN, PERI, PLIN1

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Tested Applications

• Positive WB detected in: 3T3-L1 cells, MCF-7 cells, NIH/3T3 cells, mouse liver tissue
• Positive IHC detected in: mouse brown adipose tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brown adipose tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

27716-1-AP targets Perilipin 1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, goat, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26426

  Product name: Recombinant human PLIN1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 91-200 aa of NM_001145311

  Sequence: MACRGLDHLEEKIPALQYPPEKIASELKDTISTRLRSARNSISVPIASTSDKVLGAALAGCELAWGVARDTAEFAANTRAGRLASGGADLALGSIEKVVEYLLPPDKEESA

• Full Name: perilipin
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 62-67 kDa
• GenBank Accession Number: NM_001145311
• Gene Symbol: Perilipin 1
• Gene ID (NCBI): 5346
• RRID: AB_3085989
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60240
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLIN1, also known as Perilipin 1, PLIN, PERI, belongs to the perilipin family. PLIN1 is a hormonally-regulated phosphoprotein that encircles the lipid storage droplet in adipocytes and modulates adipocyte lipid metabolism (PMID: 2040638). PLIN1 is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in inhibiting lipolysis. PLIN1 is detected in adipocytes of white adipose tissue, visceral adipose tissue, and mammary gland (PMID: 27832861, 9521880). The deficiency of PLIN1 causes lipodystrophic syndromes, which disables the optimal accumulation of triglycerides in adipocytes that results in abnormal deposition of lipids in tissues such as skeletal muscle and liver. The storage of lipids in the liver leads to insulin resistance and hypertriglyceridemia (PMID: 21345103).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB	Metabolism; Ubiquitination of cytoplasmic HMGB1 by RNF186 regulates hepatic lipophagy in non-alcoholic fatty liver disease; Authors - Jiang Du
sheep	WB	Front Vet Sci; Profile of miRNAs induced during sheep fat tail development and roles of four key miRNAs in proliferation and differentiation of sheep preadipocytes; Authors - Wei Zhang
mouse	IF	Eur J Pharmacol; SPHK2 inhibition alleviates chronic intermittent hypoxia-induced inflammation in adipose tissue by decreasing endoplasmic reticulum stress; Authors - Longyi Ran
mouse	WB	Hum Gene Ther; Kallistatin Improves High-Fat-Induced Insulin Resistance via Epididymal Adipose Tissue-Derived Exosomes; Authors - Zi-Wei Yang
mouse,human	WB	Invest Ophthalmol Vis Sci; Butyrate Ameliorates Graves' Orbitopathy Through Regulating Orbital Fibroblast Phenotypes and Gut Microbiota; Authors - Pingbo Ouyang
mouse	IF	J Plast Reconstr Aesthet Surg; Poloxamer 188 washing of lipoaspirate improves fat graft survival: A comparative study in nude mice; Authors - Meiling Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

MALLIKARJUNA (Verified Customer) (11-13-2025)

good for WB

• Applications: Western Blot
• Primary Antibody Dilution: 1;1000
• Cell Tissue Type: liver tissue