Phospho-PLK1 (Ser326) Polyclonal antibody

Phospho-PLK1 (Ser326) Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 19553-1-AP

Synonyms

Phospho-PLK1 (Ser326), phosphorylation PLK1 SER326, PLK, PLK 1, PLK1, Polo like kinase 1, STPK13



Tested Applications

Positive WB detected inMCF-7 cells, MCF7 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

19553-1-AP targets Phospho-PLK1 (Ser326) in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name polo-like kinase 1 (Drosophila)
Calculated molecular weight 68 kDa
Observed molecular weight 66 kDa
GenBank accession numberNM_005030
Gene symbol PLK1
Gene ID (NCBI) 5347
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLK1, also named as PLK and STPK13, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and CDC5/Polo subfamily. PLK1 is a Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. The antibody is specific to Ser(326)-phospho.

Protocols

Product Specific Protocols
WB protocol for Phospho-PLK1 (Ser326) antibody 19553-1-APDownload protocol
IHC protocol for Phospho-PLK1 (Ser326) antibody 19553-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Arch Biochem Biophys

Homoharringtonine inhibits melanoma cells proliferation in vitro and vivo by inducing DNA damage, apoptosis, and G2/M cell cycle arrest.

Authors - Jia-Feng Tang
ratWB

Toxicol Lett

Oxcarbazepine induces mitotic catastrophe and apoptosis in NRK-52E proximal tubular cells.

Authors - Momoka Ota