Phospho-PLK1 (Ser326) Polyclonal antibody
Phospho-PLK1 (Ser326) Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
Cat no : 19553-1-AP
|Positive WB detected in||MCF-7 cells|
|Positive IHC detected in||human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
19553-1-AP targets Phospho-PLK1 (Ser326) in WB, IHC applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||polo-like kinase 1 (Drosophila)|
|Calculated molecular weight||68 kDa|
|Observed molecular weight||66 kDa|
|GenBank accession number||NM_005030|
|Gene ID (NCBI)||5347|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PLK1, also named as PLK and STPK13, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and CDC5/Polo subfamily. PLK1 is a Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. The antibody is specific to Ser(326)-phospho.
|Product Specific Protocols|
|WB protocol for Phospho-PLK1 (Ser326) antibody 19553-1-AP||Download protocol|
|IHC protocol for Phospho-PLK1 (Ser326) antibody 19553-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Oxcarbazepine induces mitotic catastrophe and apoptosis in NRK-52E proximal tubular cells.
Arch Biochem Biophys
Homoharringtonine inhibits melanoma cells proliferation in vitro and vivo by inducing DNA damage, apoptosis, and G2/M cell cycle arrest.