Phospho-PLK1 (Ser326) Polyclonal antibody

Phospho-PLK1 (Ser326) Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 19553-1-AP


Phospho-PLK1 (Ser326), phosphorylation PLK1 SER326, PLK, PLK 1, PLK1, Polo like kinase 1, STPK13

Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

19553-1-AP targets Phospho-PLK1 (Ser326) in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name polo-like kinase 1 (Drosophila)
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberNM_005030
Gene Symbol PLK1
Gene ID (NCBI) 5347
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLK1, also named as PLK and STPK13, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and CDC5/Polo subfamily. PLK1 is a Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. The antibody is specific to Ser(326)-phospho.


Product Specific Protocols
WB protocol for Phospho-PLK1 (Ser326) antibody 19553-1-APDownload protocol
IHC protocol for Phospho-PLK1 (Ser326) antibody 19553-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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