PLK1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83260-5-RR (same clone as 83260-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83260-5-RR (PLK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83260-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83260-5-RR (same clone as 83260-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PLK1 antibody (83260-5-RR, Clone: 240092B10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83260-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 83260-5-RR (same clone as 83260-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PLK1 antibody (83260-5-RR, Clone: 240092B10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 83260-5-PBS in a different storage buffer formulation.

PLK1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 83260-5-PBS

Clone No.240092B10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

240092B10, EC:2.7.11.21, PLK, PLK 1, PLK-1

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Product Information

83260-5-PBS targets PLK1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: polo-like kinase 1 (Drosophila)
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: NM_005030
• Gene Symbol: PLK1
• Gene ID (NCBI): 5347
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P53350
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PLK1(Polo-like kinase 1), also named as PLK and STPK13, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and CDC5/Polo subfamily. PLK1 is a Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.The localization of Polo changes during the cell cycle. In interphase, Polo shows a mostly diffuse cytoplasmic localization. Polo becomes concentrated on centrosomes from early prophase and appears on centromeres or kinetochores from late prophase, before nuclear envelope breakdown (NEB). After anaphase onset, Polo relocalizes to the central spindle and remains enriched at the midbody ring in late stages of cytokinesis (PMID: 29167465).