PLOD1 Polyclonal antibody

PLOD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12475-1-AP

Synonyms

LH, LH1, LLH, Lysyl hydroxylase 1, PLOD, PLOD1



Tested Applications

Positive WB detected inFibroblasts cells, Mouse Fibroblast cells
Positive IHC detected inhuman skin cancer tissue,
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12475-1-AP targets PLOD1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, Bumblebees
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLOD1 fusion protein Ag3103
Full Name procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1
Calculated Molecular Weight 727 aa, 84 kDa
Observed Molecular Weight 85-88 kDa
GenBank Accession NumberBC016657
Gene Symbol PLOD1
Gene ID (NCBI) 5351
RRIDAB_2877860
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLOD1, also named as LLH, PLOD and LH1, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. PLOD1 catalyses the hydroxylation of lysine residues during the posttranslational modification of type I collagen, the major protein of bone.

Protocols

Product Specific Protocols
IHC protocol for PLOD1 antibody 12475-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

PLoS Genet

Cyclophilin B control of lysine post-translational modifications of skin type I collagen.

Authors - Masahiko Terajima
mouseWB,IHC

Bone

Decrease of lysyl hydroxylase 2 activity causes abnormal collagen molecular phenotypes, defective mineralization and compromised mechanical properties of bone.

Authors - Tomoaki Saito
mouseWB

Sci Rep

Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes

Authors - Masahiko Terajima
mouseWB

J Pharmacol Exp Ther

Minoxidil Cannot Be Used To Target Lysyl Hydroxylases during Postnatal Mouse Lung Development: A Cautionary Note.

Authors - Tilman Pfeffer
BumblebeesIF

Front Genet

Mating Stimulates the Immune Response and Sperm Storage-Related Genes Expression in Spermathecae of Bumblebee (Bombus terrestris) Queen.

Authors - Yueqin Guo