PLOD1 Polyclonal antibody

PLOD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 12475-1-AP

Synonyms

LH, LH1, LLH, Lysyl hydroxylase 1, PLOD, PLOD1

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Validation Data Gallery

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  WB analysis of Fibroblasts cells using 12475-1-AP

  WB result of PLOD1 antibody (12475-1-AP, 1:200) with Protein extracts from Human Fibroblasts.

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  WB analysis of Mouse Fibroblast cells using 12475-1-AP

  WB result of PLOD1 antibody (12475-1-AP, 1:250) with Osteoblasts/Fibroblasts/714 cell line Lysate.

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  IHC staining of human skin cancer using 12475-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12475-1-AP (PLOD1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin cancer using 12475-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12475-1-AP (PLOD1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin cancer using 12475-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12475-1-AP (PLOD1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 12475-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12475-1-AP (PLOD1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 12475-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12475-1-AP (PLOD1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 12475-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12475-1-AP (PLOD1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of Mouse skin papilloma using 12475-1-AP

  IHC result of PLOD1 antibody (12475-1-AP, 1:100) with mouse skin papilloma slides.

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Tested Applications

• Positive WB detected in: Fibroblasts cells, Mouse Fibroblast cells
• Positive IHC detected in: human skin cancer tissue, ; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

12475-1-AP targets PLOD1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, Bumblebees
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLOD1 fusion protein Ag3103
• Full Name: procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1
• Calculated Molecular Weight: 727 aa, 84 kDa
• Observed Molecular Weight: 85-88 kDa
• GenBank Accession Number: BC016657
• Gene Symbol: PLOD1
• Gene ID (NCBI): 5351
• RRID: AB_2877860
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLOD1, also named as LLH, PLOD and LH1, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. PLOD1 catalyses the hydroxylation of lysine residues during the posttranslational modification of type I collagen, the major protein of bone.

Publications

Species	Application	Title
mouse	WB	PLoS Genet; Cyclophilin B control of lysine post-translational modifications of skin type I collagen.; Authors - Masahiko Terajima
mouse	WB,IHC	Bone; Decrease of lysyl hydroxylase 2 activity causes abnormal collagen molecular phenotypes, defective mineralization and compromised mechanical properties of bone.; Authors - Tomoaki Saito
mouse	WB	Sci Rep; Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes; Authors - Masahiko Terajima
mouse	WB	J Pharmacol Exp Ther; Minoxidil Cannot Be Used To Target Lysyl Hydroxylases during Postnatal Mouse Lung Development: A Cautionary Note.; Authors - Tilman Pfeffer
Bumblebees	IF	Front Genet; Mating Stimulates the Immune Response and Sperm Storage-Related Genes Expression in Spermathecae of Bumblebee (Bombus terrestris) Queen.; Authors - Yueqin Guo