PLS1 Polyclonal antibody, PBS Only

• 

  WB analysis of mouse small intestine using 55212-1-AP (same clone as 55212-1-PBS)

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55212-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 55212-1-AP (same clone as 55212-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55212-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 55212-1-AP (same clone as 55212-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55212-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse small intestine using 55212-1-AP (same clone as 55212-1-PBS)

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55212-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse small intestine using 55212-1-AP (same clone as 55212-1-PBS)

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55212-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 55212-1-AP (same clone as 55212-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55212-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 55212-1-AP (same clone as 55212-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55212-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 55212-1-AP (same clone as 55212-1-PBS)

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 55212-1-AP (PLS1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55212-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 55212-1-AP (same clone as 55212-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 55212-1-AP (PLS1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55212-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 55212-1-AP (same clone as 55212-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 55212-1-AP (PLS1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55212-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PLS1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 55212-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Plastin-1, Plastin 1, I-plastin, Intestine-specific plastin, I plastin

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Product Information

55212-1-PBS targets PLS1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: plastin 1 (I isoform)
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: NM_002670
• Gene Symbol: PLS1
• Gene ID (NCBI): 5357
• RRID: AB_10859080
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14651
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PLS1, also named as I-plastin and Plastin-1, is an actin-bundling protein in the absence of calcium. It is intestine-specific plastin. This antibody is specific to PLS1. This antibody is specific to PLS1. It has no cross reaction to PLS3.