PLS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PLS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 55212-1-AP

Synonyms

Plastin-1, Plastin 1, I-plastin, Intestine-specific plastin, I plastin

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Validation Data Gallery

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  WB analysis of mouse small intestine using 55212-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 55212-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 55212-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 55212-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 55212-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 55212-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 55212-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 55212-1-AP (PLS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 55212-1-AP

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 55212-1-AP (PLS1 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 55212-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55212-1-AP (PLS1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 55212-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55212-1-AP (PLS1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse small intestine tissue, COLO 320 cells
• Positive IHC detected in: human colon cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

55212-1-AP targets PLS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: plastin 1 (I isoform)
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: NM_002670
• Gene Symbol: PLS1
• Gene ID (NCBI): 5357
• RRID: AB_10859080
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLS1, also named as I-plastin and Plastin-1, is an actin-bundling protein in the absence of calcium. It is intestine-specific plastin. This antibody is specific to PLS1. This antibody is specific to PLS1. It has no cross reaction to PLS3.

Publications

Species	Application	Title
human	WB,IHC	Cancer Sci; Plastin 1 drives metastasis of colorectal cancer through the IQGAP1/Rac1/ERK pathway.; Authors - Tongtong Zhang ; KD Validated