PLSCR1 Polyclonal antibody

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  WB analysis using 11582-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11582-1-AP

  WB result of PLSCR1 antibody (11582-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLSCR1 transfected HEK-293 cells.

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  WB analysis using 11582-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11582-1-AP

  Mouse lung lysates were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 11582-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 11582-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11582-1-AP (PLSCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 11582-1-AP

  IP result of anti-PLSCR1 (IP:11582-1-AP, 4ug; Detection:11582-1-AP 1:500) with mouse lung tissue lysate 2800ug.

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  IHC staining of mouse spleen using 11582-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11582-1-AP (PLSCR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11582-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11582-1-AP (PLSCR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 11582-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11582-1-AP (PLSCR1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11582-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11582-1-AP (PLSCR1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse spleen using 11582-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11582-1-AP (PLSCR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 11582-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using PLSCR1 antibody (11582-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of rat brain using 11582-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using PLSCR1 antibody (11582-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 11582-1-AP

  Immunofluorescent analysis of HeLa cells using 11582-1-AP (PLSCR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

PLSCR1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-Fro, IP, ELISA

Cat No. 11582-1-AP

Publications(18)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-Fro, IP, ELISA

Synonyms

Ca(2+)-dependent phospholipid scramblase 1, EC:3.1.-.-, Mg(2+)-dependent nuclease, MMTRA1B, phospholipid scramblase 1

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Tested Applications

• Positive WB detected in: HEK-293 cells, human skeletal muscle tissue, mouse skeletal muscle tissue, mouse lung tissue, A549 cells, HepG2 cells, human placenta tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: mouse spleen tissue, human kidney tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-Fro detected in: rat brain tissue, mouse brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 14 publications below
• IHC: See 5 publications below
• IF: See 8 publications below
• IP: See 2 publications below

Product Information

11582-1-AP targets PLSCR1 in WB, IHC, IF/ICC, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2172

  Product name: Recombinant human PLSCR1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-318 aa of BC021100

  Sequence: MDKQNSQMNASHPETNLPVGYPPQYPPTAFQGPPGYSGYPGPQVSYPPPPAGHSGPGPAGFPVPNQPVYNQPVYNQPVGAAGVPWMPAPQPPLNCPPGLEYLSQIDQILIHQQIELLEVLTGFETNNKYEIKNSFGQRVYFAAEDTDCCTRNCCGPSRPFTLRIIDNMGQEVITLERPLRCSSCCCPCCLQEIEIQAPPGVPIGYVIQTWHPCLPKFTIQNEKREDVLKISGPCVVCSCCGDVDFEIKSLDEQCVVGKISKHWTGILREAFTDADNFGIQFPLDLDVKMKAVMIGACFLIDFMFFESTGSQEQKSGVW

• Full Name: phospholipid scramblase 1
• Calculated Molecular Weight: 318 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC021100
• Gene Symbol: PLSCR1
• Gene ID (NCBI): 5359
• RRID: AB_2165659
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15162
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLSCR1 (Phospholipid scramblase 1) is a member of the family of membrane proteins that mediate the transbilayer movement of phospholipids ("scrambling") in a Ca2+ dependent manner, which was identified as an a type II transmembrane protein involved in the calcium-dependent, nonspecific, rapid redistribution of phospholipids. A cysteine-rich palmitoylation motif [184CCCPCC189] of PLSCR1, a thioester linkage to the sulfhydryl groups of cysteines, controls the transportation of PLSCR1 to the cell membrane or nucleus (PMID: 32292520). PLSCR1 is strongly expressed in response to interferon (IFN) treatment and viral infection (PMID: 35138119, 23487022).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	J Clin Invest; Phospholipid scramblase-1 regulates innate type 2 inflammation in mouse lung via CRTH2-dependent mechanisms; Authors - Ashley Hernandez-Gutierrez
human	WB	PLoS Pathog; Phospholipid scramblase 1 interacts with influenza A virus NP, impairing its nuclear import and thereby suppressing virus replication.; Authors - Weiyu Luo
mouse	WB	J Neurosci; Phospholipid Scramblase 1 Controls Efficient Neurotransmission and Synaptic Vesicle Retrieval at Cerebellar Synapses; Authors - Margherita Caputo ; KD Validated
human	WB,IF	J Proteome Res; An Interactome Map Reveals Phospholipid Scramblase 1 as a Novel Regulator of Hepatitis B Virus X Protein.; Authors - Yanzhi Yuan ; KD Validated
human	WB,IP,IF	Cancer Sci; Nuclear accumulation of KPNA2 impacts radioresistance through positive regulation of the PLSCR1-STAT1 loop in lung adenocarcinoma.; Authors - Wei-Chao Liao ; KD Validated
mouse	WB	J Leukoc Biol; Phospholipid scramblase 1 is required for β2-glycoprotein I binding in hypoxia and reoxygenation-induced endothelial inflammation.; Authors - Emily Archer Slone

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Teresa (Verified Customer) (01-31-2025)

The antibody gives a quite good signal in IF, however you need a strong secondary antibody for it. I still need to improve experimental setup conditions.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:30
• Cell Tissue Type: A549-ACE2, HEK293T