Tested Applications
| Positive WB detected in | HEK-293 cells, human skeletal muscle tissue, mouse skeletal muscle tissue, mouse lung tissue, A549 cells, HepG2 cells, human placenta tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | mouse spleen tissue, human kidney tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-Fro detected in | rat brain tissue, mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11582-1-AP targets PLSCR1 in WB, IHC, IF/ICC, IF-Fro, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2172 Product name: Recombinant human PLSCR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-318 aa of BC021100 Sequence: MDKQNSQMNASHPETNLPVGYPPQYPPTAFQGPPGYSGYPGPQVSYPPPPAGHSGPGPAGFPVPNQPVYNQPVYNQPVGAAGVPWMPAPQPPLNCPPGLEYLSQIDQILIHQQIELLEVLTGFETNNKYEIKNSFGQRVYFAAEDTDCCTRNCCGPSRPFTLRIIDNMGQEVITLERPLRCSSCCCPCCLQEIEIQAPPGVPIGYVIQTWHPCLPKFTIQNEKREDVLKISGPCVVCSCCGDVDFEIKSLDEQCVVGKISKHWTGILREAFTDADNFGIQFPLDLDVKMKAVMIGACFLIDFMFFESTGSQEQKSGVW Predict reactive species |
| Full Name | phospholipid scramblase 1 |
| Calculated Molecular Weight | 318 aa, 35 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC021100 |
| Gene Symbol | PLSCR1 |
| Gene ID (NCBI) | 5359 |
| RRID | AB_2165659 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15162 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PLSCR1 (Phospholipid scramblase 1) is a member of the family of membrane proteins that mediate the transbilayer movement of phospholipids ("scrambling") in a Ca2+ dependent manner, which was identified as an a type II transmembrane protein involved in the calcium-dependent, nonspecific, rapid redistribution of phospholipids. A cysteine-rich palmitoylation motif [184CCCPCC189] of PLSCR1, a thioester linkage to the sulfhydryl groups of cysteines, controls the transportation of PLSCR1 to the cell membrane or nucleus (PMID: 32292520). PLSCR1 is strongly expressed in response to interferon (IFN) treatment and viral infection (PMID: 35138119, 23487022).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PLSCR1 antibody 11582-1-AP | Download protocol |
| IHC protocol for PLSCR1 antibody 11582-1-AP | Download protocol |
| IP protocol for PLSCR1 antibody 11582-1-AP | Download protocol |
| WB protocol for PLSCR1 antibody 11582-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-PLSCR1 antibody (Cat# 11582-1-AP) has 22 citations published in journals including Nature, J Clin Invest, Cell Death Dis, PLoS Biol, PLoS Pathog, Cancer Sci, Sci Rep, J Neurosci, FEBS Lett, and PNAS. Associated research fields span virology (SARS-CoV-2, influenza A, hepatitis B/C, HIV-1), oncology (colorectal, glioma, TNBC, tongue squamous cell carcinoma), immunology and inflammation, neuroscience, glaucoma pathogenesis, and placental biology.
| Species | Application | Title |
|---|---|---|
Nature PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection
| ||
Genes Dis PLSCR1 promotes apoptosis and clearance of retinal ganglion cells in glaucoma pathogenesis | ||
J Clin Invest Phospholipid scramblase-1 regulates innate type 2 inflammation in mouse lung via CRTH2-dependent mechanisms | ||
Cell Death Dis PLSCR1 drives chemoresistance in TNBC via METTL3/IGF2BP3-mediated mRNA stabilization and EGFR-MAPK pathway activation. | ||
Mol Med Identification of phospholipid scramblase 1 as a biomarker and determination of its prognostic value for colorectal cancer. | ||
PLoS Biol A genome-wide arrayed CRISPR screen identifies PLSCR1 as an intrinsic barrier to SARS-CoV-2 entry that recent virus variants have evolved to resist |
Reviews
What customers say
One user (Teresa L.) rated this antibody 3/5 for immunofluorescence on A549-ACE2 and HEK293T cells at a 1:30 dilution. She reported that the antibody gives a quite good signal in IF but requires a strong secondary antibody to achieve optimal results. She noted she still needed to improve her experimental setup conditions. Overall, the feedback is moderately positive with room for optimization in protocol refinement.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Teresa (Verified Customer) (01-31-2025) | The antibody gives a quite good signal in IF, however you need a strong secondary antibody for it. I still need to improve experimental setup conditions.
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