Anti-Human PLXNC1 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98534-2-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human PLXNC1 Rabbit RecAb (98534-2-RR, Clone: 250374F2) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD45 Rabbit Recombinant Antibody (APC-98117, Clone: 241670E10). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98534-2-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human PLXNC1 Rabbit RecAb (98534-2-RR, Clone: 250374F2) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  FC experiment of Transfected CHO using 98534-2-RR

  1x10^6 CHO cells or PLXNC1 transfected CHO were surface stained with 0.25 ug Anti-Human PLXNC1 Rabbit RecAb (98534-2-RR, Clone: 250374F2) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed.

• 

  FC experiment of Transfected CHO using 98534-2-RR

  1x10^6 CHO cells or PLXNC1 transfected CHO were surface stained with 0.25 ug Anti-Human PLXNC1 Rabbit RecAb (98534-2-RR, Clone: 250374F2) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98534-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98534-2-RR against Human PLXNC1 were performed. The affinity constant is 32.0 pM.

PLXNC1 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98534-2-RR

Clone No.250374F2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD232, plexin C1, PLXN C1, VESPR

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Tested Applications

• Positive FC detected in: human PBMCs, Transfected CHO cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98534-2-RR targets PLXNC1 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2721

  Product name: Recombinant Human PLXNC1 protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 35-944 aa of NM_005761.3

  Sequence: ADEPVWRSEQAIGAIAASQEDGVFVASGSCLDQLDYSLEHSLSRLYRDQAGNCTEPVSLAPPARPRPGSSFSKLLLPYREGAAGLGGLLLTGWTFDRGACEVRPLGNLSRNSLRNGTEVVSCHPQGSTAGVVYRAGRNNRWYLAVAATYVLPEPETASRCNPAASDHDTAIALKDTEGRSLATQELGRLKLCEGAGSLHFVDAFLWNGSIYFPYYPYNYTSGAATGWPSMARIAQSTEVLFQGQASLDCGHGHPDGRRLLLSSSLVEALDVWAGVFSAAAGEGQERRSPTTTALCLFRMSEIQARAKRVSWDFKTAESHCKEGDQPERVQPIASSTLIHSDLTSVYGTVVMNRTVLFLGTGDGQLLKVILGENLTSNCPEVIYEIKEETPVFYKLVPDPVKNIYIYLTAGKEVRRIRVANCNKHKSCSECLTATDPHCGWCHSLQRCTFQGDCVHSENLENWLDISSGAKKCPKIQIIRSSKEKTTVTMVGSFSPRHSKCMVKNVDSSRELCQNKSQPNRTCTCSIPTRATYKDVSVVNVMFSFGSWNLSDRFNFTNCSSLKECPACVETGCAWCKSARRCIHPFTACDPSDYERNQEQCPVAVEKTSGGGRPKENKGNRTNQALQVFYIKSIEPQKVSTLGKSNVIVTGANFTRASNITMILKGTSTCDKDVIQVSHVLNDTHMKFSLPSSRKEMKDVCIQFDGGNCSSVGSLSYIALPHCSLIFPATTWISGGQNITMMGRNFDVIDNLIISHELKGNINVSEYCVATYCGFLAPSLKSSKVRTNVTVKLRVQDTYLDCGTLQYREDPRFTGYRVESEVDTELEVKIQKENDNFNISKKDIEITLFHGENGQLNCSFENITRNQDLTTILCKIKGIKTASTIANSSKKVRVKLGNLELYVEQESVPST

• Full Name: plexin C1
• Calculated Molecular Weight: 176 kDa
• GenBank Accession Number: NM_005761.3
• Gene Symbol: PLXNC1
• Gene ID (NCBI): 10154
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O60486
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

PLXNC1 (Plexin-C1) is a semaphorin receptor that bridges extracellular guidance cues to intracellular GTPase signaling, influencing cell adhesion, migration, and immune modulation. Predominantly expressed in neuronal, epithelial, and immune tissues, PLXNC1 regulates axon guidance and inflammatory balance via SEMA7A-dependent pathways. Dysregulated PLXNC1 signaling contributes to cancer progression, immune dysregulation, and neuronal pathologies, highlighting its versatile role in cell communication and morphogenesis.