PMP2 Polyclonal antibody

PMP2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 12717-1-AP

Synonyms

FABP8, M FABP, MP2, Myelin P2 protein, P2, peripheral myelin protein 2, PMP2

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Validation Data Gallery

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  WB analysis of pig spinal cord using 12717-1-AP

  pig spinal cord tissue were subjected to SDS PAGE followed by western blot with 12717-1-AP (PMP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12717-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12717-1-AP (PMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12717-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12717-1-AP (PMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 12717-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12717-1-AP (PMP2 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: pig spinal cord tissue, human brain tissue, mouse testis tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Product Information

12717-1-AP targets PMP2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PMP2 fusion protein Ag3411
• Full Name: peripheral myelin protein 2
• Calculated molecular weight: 132 aa, 15 kDa
• Observed molecular weight: 15-20 kDa
• GenBank accession number: BC034997
• Gene symbol: PMP2
• Gene ID (NCBI): 5375
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PMP2 (peripheral myelin protein-2, also known as P2) is one of the major proteins of peripheral myelin and appears to be related to the transport of fatty acids or the metabolism of myelin lipids.P2 constitutes up to 15% of total protein of peripheral nervous system (PNS) myelin. It is also present in small amounts in central nervous system (CNS) myelin, being abundant in spinal cord and brain stem myelin. As a structural protein, P2 is thought to stabilize the myelin membranes.

Publications

Species	Application	Title
human	IHC	Nat Neurosci; Variation among intact tissue samples reveals the core transcriptional features of human CNS cell classes.; Authors - Kevin W Kelley
mouse	IHC	Nat Neurosci; Disentangling glial diversity in peripheral nerves at single-nuclei resolution.; Authors - Aldrin K Y Yim
pig	WB	Neuron; Macrophages Expressing GALC Improve Peripheral Krabbe Disease by a Mechanism Independent of Cross-Correction.; Authors - Nadav I Weinstock
mouse	WB	Elife; Proteome profile of peripheral myelin in healthy mice and in a neuropathy model.; Authors - Sophie B Siems
human,mouse	WB,IHC	Elife; Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice.; Authors - Vasiliki-Ilya Gargareta
mouse	IHC	Glia; A role of peripheral myelin protein 2 in lipid homeostasis of myelinating schwann cells.; Authors - Jennifer Zenker