POLA2 Polyclonal antibody, PBS Only

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  WB analysis of HepG2 using 21778-1-AP (same clone as 21778-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 21778-1-AP (same clone as 21778-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 21778-1-AP (same clone as 21778-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 21778-1-AP (same clone as 21778-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 21778-1-AP (same clone as 21778-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 21778-1-AP (same clone as 21778-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 21778-1-AP (same clone as 21778-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 21778-1-AP (same clone as 21778-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 21778-1-AP (same clone as 21778-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21778-1-AP (POLA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.

POLA2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 21778-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

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Product Information

21778-1-PBS targets POLA2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0271

  Product name: Recombinant human POLA2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 17-275 aa of BC001347

  Sequence: LDCEEALIEKLVELCVQYGQNEEGMVGELIAFCTSTHKVGLTSEILNSFEHEFLSKRLSKARHSTCKDSGHAGARDIVSIQELIEVEEEEEILLNSYTTPSKGSQKRAISTPETPLTKRSVSTRSPHQLLSPSSFSPSATPSQKYNSRSNRGEVVTSFGLAQGVSWSGRGGAGNISLKVLGCPEALTGSYKSMFQKLPDIREVLTCKIEELGSELKEHYKIEAFTPLLAPAQEPVTLLGQIGCDSNGKLNNKSVILEGD

• Full Name: polymerase (DNA directed), alpha 2 (70kD subunit)
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 68-70 kDa
• GenBank Accession Number: BC001347
• Gene Symbol: POLA2
• Gene ID (NCBI): 23649
• RRID: AB_10733243
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14181
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.