POLA2 antibody (21778-1-PBS)

Filter: AllWBIF/ICC

WB analysis of HepG2 using 21778-1-AP (same clone as 21778-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 21778-1-AP (same clone as 21778-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.
WB analysis of COLO 320 using 21778-1-AP (same clone as 21778-1-PBS)
COLO 320 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 21778-1-AP (same clone as 21778-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 21778-1-AP (same clone as 21778-1-PBS)
MCF7 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 21778-1-AP (same clone as 21778-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.
WB analysis of COLO 320 using 21778-1-AP (same clone as 21778-1-PBS)
COLO 320 cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 21778-1-AP (same clone as 21778-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 21778-1-AP (POLA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 21778-1-AP (same clone as 21778-1-PBS)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21778-1-AP (POLA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21778-1-PBS in a different storage buffer formulation.

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Tested Applications
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Product Information

21778-1-PBS targets POLA2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	POLA2 fusion protein Ag0271 Predict reactive species
Full Name	polymerase (DNA directed), alpha 2 (70kD subunit)
Calculated Molecular Weight	70 kDa
Observed Molecular Weight	68-70 kDa
GenBank Accession Number	BC001347
Gene Symbol	POLA2
Gene ID (NCBI)	23649
RRID	AB_10733243
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14181
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.