POLD1 Polyclonal antibody, PBS Only

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  WB analysis using 15646-1-AP (same clone as 15646-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15646-1-AP (POLD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 15646-1-AP (same clone as 15646-1-PBS)

  IP result of anti-POLD1 (IP:15646-1-AP, 4ug; Detection:15646-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15646-1-AP (same clone as 15646-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15646-1-AP (POLD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15646-1-AP (same clone as 15646-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15646-1-AP (POLD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 15646-1-AP (same clone as 15646-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15646-1-AP (POLD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 15646-1-AP (same clone as 15646-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15646-1-AP (POLD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

POLD1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 15646-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

15646-1-PBS targets POLD1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8178

  Product name: Recombinant human POLD1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 858-1107 aa of BC008800

  Sequence: EGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVLSHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRFGPPGPEAW

• Full Name: polymerase (DNA directed), delta 1, catalytic subunit 125kDa
• Calculated Molecular Weight: 1107 aa, 124 kDa
• Observed Molecular Weight: 124 kDa
• GenBank Accession Number: BC008800
• Gene Symbol: POLD1
• Gene ID (NCBI): 5424
• RRID: AB_2252572
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P28340
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

POLD1, also known as DNA polymerase subunit delta p125, is 1107 amino acid protein, which belongs to the DNA polymerase type-B family. POLD1 colocalizes with PCNA and POLD3 at S phase replication sites (PubMed:11595739). As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. A previous study demonstrated that overexpression of POLD1 is associated with an increased risk of breast cancer. (PMID: 30344713 )