PPAT Polyclonal antibody

• Featured Product
• KD/KO Validated

PPAT Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 15401-1-AP

Synonyms

Amidophosphoribosyltransferase, ATASE, GPAT, PPAT, PRAT

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Validation Data Gallery

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  WB analysis of HEK-293 using 15401-1-AP

  WB result of PPAT antibody (15401-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPAT transfected HEK-293 cells.

• 

  WB analysis using 15401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15401-1-AP (PPAT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15401-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15401-1-AP (PPAT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15401-1-AP

  IP result of anti-PPAT (IP:15401-1-AP, 4ug; Detection:15401-1-AP 1:500) with HepG2 cells lysate 2400ug.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells
• Positive IP detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 24 publications below
• IHC: See 1 publications below

Product Information

15401-1-AP targets PPAT in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPAT fusion protein Ag7618
• Full Name: phosphoribosyl pyrophosphate amidotransferase
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 50-56 kDa
• GenBank Accession Number: BC004200
• Gene Symbol: PPAT
• Gene ID (NCBI): 5471
• RRID: AB_2166532
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
		Science; mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.; Authors - Issam Ben-Sahra
human	WB	Cancer Cell; Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming.; Authors - Yoichiro Mitsuishi
human	WB	Mol Cell; ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.; Authors - Eunus S Ali
human	WB	Nat Commun; A shift in glutamine nitrogen metabolism contributes to the malignant progression of cancer.; Authors - Manabu Kodama
human	WB	Nat Commun; Menin enhances c-Myc-mediated transcription to promote cancer progression.; Authors - Gongwei Wu
human	WB	EMBO J; UHMK1 promotes gastric cancer progression through reprogramming nucleotide metabolism.; Authors - Xing Feng ; KD Validated

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