PPM1H Polyclonal antibody

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  WB analysis using 13767-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13767-1-AP (PPM1H antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

PPM1H Polyclonal Antibody for WB, ELISA

Cat No. 13767-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ARHCL1, EC:3.1.3.16, KIAA1157, Protein phosphatase 1H, URCC2

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13767-1-AP targets PPM1H in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4750

  Product name: Recombinant human PPM1H protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-82 aa of BC040177

  Sequence: MLTRVKSAVANFMGGIMAGSSGSEHGGGSCGGSDLPLRFPYGRPEFLGLSQDEVECSADHIARPILILKETRRLPWATGYAE

• Full Name: protein phosphatase 1H (PP2C domain containing)
• Calculated Molecular Weight: 82 aa, 9 kDa
• Observed Molecular Weight: 50-56 kDa
• GenBank Accession Number: BC040177
• Gene Symbol: PPM1H
• Gene ID (NCBI): 57460
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9ULR3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPM1H (Protein phosphatase 1H ) is a member of a group of 3 PPM phosphatases that have been termed the 'PPM1H subfamily. In blood cells, PPM1H is expressed at high levels in basophils and lower levels in neutrophils and is very low or not detected in most other cells including monocytes. PPM1H is localized to the Golgi and its knockdown suppresses primary cilia formation, similar to pathogenic LRRK2 (PMID: 27599670, 31663853)