PPM1K Polyclonal antibody, PBS Only

• 

  WB analysis of human brain using 14573-1-AP (same clone as 14573-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 14573-1-AP (PPM1K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14573-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 14573-1-AP (same clone as 14573-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 14573-1-AP (PPM1K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14573-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 14573-1-AP (same clone as 14573-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14573-1-AP (PPM1K antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14573-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 14573-1-AP (same clone as 14573-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14573-1-AP (PPM1K antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14573-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PPM1K Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 14573-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

14573-1-PBS targets PPM1K in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5660

  Product name: Recombinant human PPM1K protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-372 aa of BC037552

  Sequence: MSTAALITLVRSGGNQVRRRVLLSSRLLQDDRRVTPTCHSSTSEPRCSRFDPDGSGSPATWDNFGIWDNRIDEPILLPPSIKYGKPIPKISLEKVGCASQIGKRKENEDRFDFAQLTDEVLYFAVYDGHGGPAAADFCHTHMEKCIMDLLPKEKNLETLLTLAFLEIDKAFSSHARLSADATLLTSGTTATVALLRDGIELVVASVGDSRAILCRKGKPMKLTIDHTPERKDEKERIKKCGGFVAWNSLGQPHVNGRLAMTRSIGDLDLKTSGVIAEPETKRIKLHHADDSFLVLTTDGINFMVNSQEICDFVNQCHDPNEAAHAVTEQAIQYGTEDNSTAVVVPFGAWGKYKNSEINFSFSRSFASSGRWA

• Full Name: protein phosphatase 1K (PP2C domain containing)
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: BC037552
• Gene Symbol: PPM1K
• Gene ID (NCBI): 152926
• RRID: AB_2252953
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N3J5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Protein phosphatase, Mg2+/Mn2+ dependent 1K (PPM1K), is a member of the PPM family of Mn2+/Mg2+-dependent protein phosphatases. PPMiK, essential for cell survival and development, is targeted to the mitochondria where it plays a key role in regulation of the mitochondrial permeability transition pore.PPM1K is essential for the regulation and nutrient-induced activation of the branched-chain alpha-keto acid dehydrogenase complex (BCKD or BCKDC), which catalyzes the breakdown of branched-chain amino acids (BCAA).