PPM1K Polyclonal antibody

• Featured Product
• KD/KO Validated

PPM1K Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (2)

Cat no : 14573-1-AP

Synonyms

PP2C kappa, PP2Cm, PPM1K, PTMP, UG0882E07

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Validation Data Gallery

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  WB analysis of human brain using 14573-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14573-1-AP (PPM1K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14573-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14573-1-AP (PPM1K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 14573-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14573-1-AP (PPM1K antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 14573-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14573-1-AP (PPM1K antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human brain tissue, human heart tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 1 publications below

Product Information

14573-1-AP targets PPM1K in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPM1K fusion protein Ag5660
• Full Name: protein phosphatase 1K (PP2C domain containing)
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: BC037552
• Gene Symbol: PPM1K
• Gene ID (NCBI): 152926
• RRID: AB_2252953
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein phosphatase, Mg2+/Mn2+ dependent 1K (PPM1K), is a member of the PPM family of Mn2+/Mg2+-dependent protein phosphatases. PPMiK, essential for cell survival and development, is targeted to the mitochondria where it plays a key role in regulation of the mitochondrial permeability transition pore.PPM1K is essential for the regulation and nutrient-induced activation of the branched-chain alpha-keto acid dehydrogenase complex (BCKD or BCKDC), which catalyzes the breakdown of branched-chain amino acids (BCAA).

Publications

Species	Application	Title
human,mouse	WB,IF	Nat Commun; Glucose promotes cell growth by suppressing branched-chain amino acid degradation.; Authors - Dan Shao ; KO Validated
mouse	WB	Free Radic Biol Med; Elevated branched-chain α-keto acids exacerbate macrophage oxidative stress and chronic inflammatory damage in type 2 diabetes mellitus.; Authors - Shuyun Liu
mouse	WB	Front Pharmacol; Pyridostigmine Protects Against Diabetic Cardiomyopathy by Regulating Vagal Activity, Gut Microbiota, and Branched-Chain Amino Acid Catabolism in Diabetic Mice.; Authors - Yang Yang
rat	WB	PLoS One; KIC (ketoisocaproic acid) and leucine have divergent effects on tissue insulin signaling but not on whole-body insulin sensitivity in rats; Authors - Gagandeep Mann
rat	WB	Physiol Rep; Downregulation of extramitochondrial BCKDH and its uncoupling from AMP deaminase in type 2 diabetic OLETF rat hearts; Authors - Toshifumi Ogawa
rat	WB	Am J Physiol Cell Physiol; Maintenance of the Branched-Chain Amino Acid transporter LAT1 Counteracts Myotube Atrophy following Chemotherapy; Authors - Stephen Mora

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

CHAO (Verified Customer) (01-30-2020)

Clear, sharp band as predicted size 40 kDa.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

FH

Lorenza (Verified Customer) (11-08-2019)

I have tried four different antibodies from different companies and this is the only one that works in total lysate (and not only in isolated mitochondria). All the others give an unspecific band at the same molecular weight. Works perfectly. There are some unspecific bands at higher molecular weights but the right bands is easily detectable.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: total heart lysate and total liver lysate

PPM1K Antibody Western Blot, validation (1:1000 dilution) in total heart lysate and total liver lysate (Cat no:14573-1-AP)