PPP1R17 Polyclonal antibody

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  WB analysis using 27976-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27976-1-AP (C7orf16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 27976-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27976-1-AP (PPP1R17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 27976-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PPP1R17 antibody (27976-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U2OS using 27976-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PPP1R17 antibody (27976-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PPP1R17 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 27976-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C7orf16, G substrate, GSBS, G-substrate, Protein phosphatase 1 regulatory subunit 17

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Tested Applications

• Positive WB detected in: mouse cerebellum tissue, rat cerebellum tissue
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27976-1-AP targets PPP1R17 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15187

  Product name: Recombinant human C7orf16 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-155 aa of BC028094

  Sequence: MMSTEQMQPLEVSEDRLDKLDPRCSHLDDLSDQFIKDCDLKKKPRKGKNVQATLNVESDQKKPRRKDTPALHIPPFIPGVFSEHLIKRYDVQERHPKGKMIPVLHNTDLEQKKPRRKDTPALHMSPFAAGVTLLRDERPKAIVEDDEKDGDKIAI

• Full Name: chromosome 7 open reading frame 16
• Calculated Molecular Weight: 155 aa, 18 kDa
• Observed Molecular Weight: 20-30 kDa
• GenBank Accession Number: BC028094
• Gene Symbol: PPP1R17
• Gene ID (NCBI): 10842
• RRID: AB_3086018
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O96001
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP1R17, an endogenous substrate for cGMP-dependent protein kinase, exists almost exclusively in cerebellar Purkinje cells, where it is possibly involved in the induction of long-term depression (PMID: 10051666). PPP1R17 inhibits phosphatase activities of protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A) complexes. Allelic variants increase susceptibility to hypercholesterolemia (PMID: 12955585).