PRDM8 Polyclonal antibody

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  WB analysis using 26010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26010-1-AP (PRDM8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26010-1-AP (PRDM8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26010-1-AP (PRDM8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of hTERT-RPE1 using 26010-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using PRDM8 antibody (26010-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

PRDM8 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 26010-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

EC:2.1.1.-, PR domain containing 8, PR domain containing protein 8, PR domain-containing protein 8

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Tested Applications

• Positive WB detected in: Neuro-2a cells, mouse testis tissue, mouse retina tissue, rat testis tissue
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26010-1-AP targets PRDM8 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23373

  Product name: Recombinant human PRDM8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC071584

  Sequence: MEDTGIQRGIWDGDAKAVQQCLTDIFTSVYTTCDIPENAIFGPCVLSHTSLYDSIAFIALKSTDKRTVPYIFRVDTSAANGSSEGLMWLRLVQSARDKEEQNLEAYIKNGQLFYRSLRRIAKDEELLVWYGKELTELLLLCPSRSHNKMNGSSPYTCLECSQRFQFEFPYVAHLRFRCPKRLHSADISPQDEQGGGVGTKDHGGGGGGGKDQQQQQQEAPLGPGPKFCKAGPLHHYPSPSPESSNPSAAAGGSSAKPSTDFHNLARELENSRGGSSCSPAQSLSSGSGSGGGGGHQEAELSPDGIATGGGKGKRKFPEEAAEGGGGAGLVGGRGRFVERPLPASKEDLVC

• Full Name: PR domain containing 8
• Calculated Molecular Weight: 689 aa, 72 kDa
• Observed Molecular Weight: 35 kDa, 72 kDa
• GenBank Accession Number: BC071584
• Gene Symbol: PRDM8
• Gene ID (NCBI): 56978
• RRID: AB_3669516
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NQV8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDI-BF1 and RIZ homology domain zinc fnger protein 8 (PRDM8) belongs to the PRDM family characterized by a conserved N-terminal PR domain related to the catalytic SET domains. PRDM8 is a key regulator in neural development and testis steroidogenesis (PMID: 32819411). PRDM8 is mainly demonstrated as a crucial regulatory molecule during neural development and testis steroidogen and expressed at low levels in HCC tissues (PMID: 29572888).