PRDX6 Monoclonal antibody

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• PRDX6 Monoclonal antibody (67499-1-Ig)

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PRDX6 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 67499-1-Ig Copied!

Clone No. 3C12D3

Publications (3)

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Host / Isotype

Mouse / IgG2a

Reactivity

human, pig and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA
For FC, select conjugated version.

Synonyms

1-Cys peroxiredoxin, 1-Cys PRX, 3C12D3, Acidic calcium-independent phospholipase A2, AOP2

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  WB analysis using 67499-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67499-1-Ig (PRDX6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HAP1 and HeLa using 67499-1-Ig

  HAP1 and HeLa (WT and PRDX6 KO) lysates prepared with RIPA buffer, 25ug protein loaded. 67499-1-Ig incubated at 1:1000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis of HeLa using 67499-1-Ig

  WB result of PRDX6 antibody (67499-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX6 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 67499-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67499-1-Ig (PRDX6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HAP1 using 67499-1-Ig

  HAP1 lysates prepared and IP of peroxidoxin 6 performed using 2.0 μg of 67499-1-Ig coupled to protein G- Sepharose beads. Ponceau stained transfers shown for each blot. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human liver using 67499-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67499-1-Ig (PRDX6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human liver using 67499-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67499-1-Ig (PRDX6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 67499-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRDX6 antibody (67499-1-Ig, Clone: 3C12D3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HAP1 using 67499-1-Ig

  HAP1 WT cells (yellow outline) and PRDX6 KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 67499-1-lg at 1:1000 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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Tested Applications

• Positive WB detected in: LNCaP cells, HAP1 cells, A549 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, pig brain tissue, HepG2 cells
• Positive IP detected in: HAP1 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HAP1

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67499-1-Ig targets PRDX6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4727

  Product name: Recombinant human PRDX6 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-224 aa of BC035857

  Sequence: MPGGLLLGDVAPNFEANTTVGRIRFHDFLGDSWGILFSHPRDFTPVCTTELGRAAKLAPEFAKRNVKLIALSIDSVEDHLAWSKDINAYNCEEPTEKLPFPIIDDRNRELAILLGMLDPAEKDEKGMPVTARVVFVFGPDKKLKLSILYPATTGRNFDEILRVVISLQLTAEKRVATPVDWKDGDSVMVLPTIPEEEAKKLFPKGVFTKELPSGKKYLRYTPQP

• Full Name: peroxiredoxin 6
• Calculated Molecular Weight: 224 aa, 25 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC035857
• Gene Symbol: PRDX6
• Gene ID (NCBI): 9588
• RRID: AB_2882723
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P30041
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).

Publications

What published studies show

The anti-PRDX6 antibody (Cat# 67499-1-Ig) has 3 total citations published in Redox Reports, Reproductive Biology and Endocrinology, and iScience. Associated research fields include ferroptosis and sepsis-associated acute kidney injury, oxidative stress in asthenozoospermic sperm, and microprotein identification in cancer cell fitness. Applications cited include Western blot and immunofluorescence, with reactivity in mouse, human, and unspecified species.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB	Redox Rep; Sirtuin 5-mediated desuccinylation of PRDX6 inhibits ferroptosis and alleviates sepsis-associated acute kidney injury.; Authors - Wenlong Lin
human	IF	Reprod Biol Endocrinol; Semenogelin I-derived peptide SgI-52 binds membrane Peroxiredoxin 6 and induces oxidative stress in asthenozoospermic sperm.; Authors - Yanting Yang
		iScience; A large-scale sORF screen identifies putative microproteins involved in cancer cell fitness; Authors - Dörte Schlesinger