PRDX6 Monoclonal antibody, PBS Only

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  WB analysis using 67499-1-Ig (same clone as 67499-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67499-1-Ig (PRDX6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67499-1-PBS in a different storage buffer formulation.

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  WB analysis of HAP1 and HeLa using 67499-1-Ig (same clone as 67499-1-PBS)

  HAP1 and HeLa (WT and PRDX6 KO) lysates prepared with RIPA buffer, 25ug protein loaded. 67499-1-Ig incubated at 1:1000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 67499-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67499-1-Ig (same clone as 67499-1-PBS)

  WB result of PRDX6 antibody (67499-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX6 transfected HeLa cells. This data was developed using the same antibody clone with 67499-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67499-1-Ig (same clone as 67499-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67499-1-Ig (PRDX6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67499-1-PBS in a different storage buffer formulation.

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  IP experiment of HAP1 using 67499-1-Ig (same clone as 67499-1-PBS)

  HAP1 lysates prepared and IP of peroxidoxin 6 performed using 2.0 μg of 67499-1-Ig coupled to protein G- Sepharose beads. Ponceau stained transfers shown for each blot. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 67499-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67499-1-Ig (same clone as 67499-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67499-1-Ig (PRDX6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67499-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67499-1-Ig (same clone as 67499-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67499-1-Ig (PRDX6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67499-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67499-1-Ig (same clone as 67499-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRDX6 antibody (67499-1-Ig, Clone: 3C12D3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67499-1-PBS in a different storage buffer formulation.

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  IF Staining of HAP1 using 67499-1-Ig (same clone as 67499-1-PBS)

  HAP1 WT cells (yellow outline) and PRDX6 KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 67499-1-lg at 1:1000 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 67499-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PRDX6 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 67499-1-PBS

Clone No.3C12D3

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

1-Cys peroxiredoxin, 1-Cys PRX, 3C12D3, Acidic calcium-independent phospholipase A2, AOP2

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Product Information

67499-1-PBS targets PRDX6 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4727

  Product name: Recombinant human PRDX6 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-224 aa of BC035857

  Sequence: MPGGLLLGDVAPNFEANTTVGRIRFHDFLGDSWGILFSHPRDFTPVCTTELGRAAKLAPEFAKRNVKLIALSIDSVEDHLAWSKDINAYNCEEPTEKLPFPIIDDRNRELAILLGMLDPAEKDEKGMPVTARVVFVFGPDKKLKLSILYPATTGRNFDEILRVVISLQLTAEKRVATPVDWKDGDSVMVLPTIPEEEAKKLFPKGVFTKELPSGKKYLRYTPQP

• Full Name: peroxiredoxin 6
• Calculated Molecular Weight: 224 aa, 25 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC035857
• Gene Symbol: PRDX6
• Gene ID (NCBI): 9588
• RRID: AB_2882723
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P30041
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).