PRKAR1B Polyclonal antibody

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  WB analysis of mouse brain using 17991-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17991-1-AP (PRKAR1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17991-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17991-1-AP (PRKAR1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 17991-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 17991-1-AP (PRKAR1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 17991-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17991-1-AP (PRKAR1B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17991-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17991-1-AP (PRKAR1B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17991-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17991-1-AP (PRKAR1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17991-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17991-1-AP (PRKAR1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17991-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17991-1-AP (PRKAR1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PRKAR1B Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17991-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

cAMP-dependent protein kinase type I-beta regulatory subunit, PRKAR1

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Tested Applications

• Positive WB detected in: mouse brain tissue, Transfected HEK-293 cells, HeLa cells
• Positive IHC detected in: human breast cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

17991-1-AP targets PRKAR1B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12426

  Product name: Recombinant human PRKAR1B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-381 aa of BC026734

  Sequence: MASPPACPSEEDESLKGCELYVQLHGIQQVLKDCIVHLCISKPERPMKFLREHFEKLEKEENRQILARQKSNSQSDSHDEEVSPTPPNPVVKARRRRGGVSAEVYTEEDAVSYVRKVIPKDYKTMTALAKAISKNVLFAHLDDNERSDIFDAMFPVTHIAGETVIQQGNEGDNFYVVDQGEVDVYVNGEWVTNISEGGSFGELALIYGTPRAATVKAKTDLKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLEKWERLTVADALEPVQFEDGEKIVVQGEPGDDFYIITEGTASVLQRRSPNEEYVEVGRLGPSDYFGEIALLLNRPRAATVVARGPLKCVKLDRPRFERVLGPCSEILKRNIQRYNSFISLTV

• Full Name: protein kinase, cAMP-dependent, regulatory, type I, beta
• Calculated Molecular Weight: 381 aa, 43 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC026734
• Gene Symbol: PRKAR1B
• Gene ID (NCBI): 5575
• RRID: AB_2878478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P31321
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Front Immunol; PRKAR1B as an oncogenic biomarker for diagnostic and prognostic stratification of tumor immunity, proliferation, and migration in head and neck squamous cell carcinoma.; Authors - Peng Zhao ; KD Validated