Multi-rAb® PRMT5 Multi-Recombinant antibody

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• Multi-rAb® PRMT5 Multi-Recombinant antibody (RMX00051)

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  WB analysis using RMX00051

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00051 (PRMT5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using RMX00051

  IP result of anti-PRMT5 (IP:RMX00051, 4ug; Detection:RMX00051 1:10000) with NIH/3T3 cells lysate 1960 ug.

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  IHC staining of mouse brain using RMX00051

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00051 (PRMT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using RMX00051

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00051 (PRMT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using RMX00051

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using RMX00051 (PRMT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using RMX00051

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRMT5 antibody (RMX00051) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

PRMT5 Multi-Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. RMX00051

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

72 kDa ICln-binding protein, EC:2.1.1.320, Histone-arginine N-methyltransferase PRMT5, Jak-binding protein 1, Protein arginine N methyltransferase 5

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Tested Applications

• Positive WB detected in: HepG2 cells, Raji cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: NIH/3T3 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00051 targets PRMT5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: protein arginine methyltransferase 5
• Calculated Molecular Weight: 637 aa, 73 kDa
• Observed Molecular Weight: 68-72 kDa
• GenBank Accession Number: BC025979
• Gene Symbol: PRMT5
• Gene ID (NCBI): 10419
• RRID: AB_3750103
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: O14744
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRMT5 is a Type II enzyme that catalyzes arginine monomethylation and symmetric dimethylation (Rme1 and Rme2s). The many targets of PRMT5 include ribosomal proteins, the histone chaperone nucleoplasmin, p53, and histones. PRMT5 is frequently observed in a complex with the cofactor, methylosome protein 50 (MEP50), which is required for PRMT5 activity. PRMT5 is upregulated in several human malignancies, including lymphomas, lung cancer, breast cancer and colorectal cancer.