PRMT7 Monoclonal antibody, PBS Only

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  WB analysis using 67669-1-Ig (same clone as 67669-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67669-1-Ig (PRMT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67669-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 67669-1-Ig (same clone as 67669-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67669-1-Ig (PRMT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67669-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67669-1-Ig (same clone as 67669-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67669-1-Ig (PRMT7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67669-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 67669-1-Ig (same clone as 67669-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PRMT7 antibody (67669-1-Ig, Clone: 3C5H6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67669-1-PBS in a different storage buffer formulation.

PRMT7 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 67669-1-PBS

Clone No.3C5H6

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

FLJ10640, KIAA1933, PRMT7

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Product Information

67669-1-PBS targets PRMT7 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30434

  Product name: Recombinant human PRMT7 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 353-693 aa of BC000146

  Sequence: PEKNERVRQMRPVCDCQAHLLWNRPRFGEINDQDRTDRYVQALRTVLKPDSVCLCVSDGSLLSVLAHHLGVEQVFTVESSAASHKLLRKIFKANHLEDKINIIEKRPELLTNEDLQGRKVSLLLGEPFFTTSLLPWHNLYFWYVRTAVDQHLGPGAMVMPQAASLHAVVVEFRDLWRIRSPCGDCEGFDVHIMDDMIKRALDFRESREAEPHPLWEYPCRSLSEPWQILTFDFQQPVPLQPLCAEGTVELRRPGQSHAAVLWMEYHLTPECTLSTGLLEPADPEGGCCWNPHCKQAVYFFSPAPDPRALLGGPRTVSYAVEFHPDTGDIIMEFRHADTPD

• Full Name: protein arginine methyltransferase 7
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 78 kDa
• GenBank Accession Number: BC000146
• Gene Symbol: PRMT7
• Gene ID (NCBI): 54496
• RRID: AB_2882865
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NVM4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Protein arginine methyltransferases (PRMTs) are responsible for arginine methylation of histone or nonhistone proteins to regulate diverse biological processes, including cell proliferation, differentiation and senescence. PRMT7 is expressed in both embryonic stem and germ cells and is implicated in maintenance of muscle stem cell function. overexpression of PRMT7 has been associated with cancer metastasis. PRMT7 automethylation at residue R531 was reported to enhance breast cancer metastasis.