PRMT7 Monoclonal antibody

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  WB analysis using 67669-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67669-1-Ig (PRMT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67669-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67669-1-Ig (PRMT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67669-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67669-1-Ig (PRMT7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 67669-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PRMT7 antibody (67669-1-Ig, Clone: 3C5H6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

PRMT7 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67669-1-Ig

Clone No.3C5H6

Publications(2)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

FLJ10640, KIAA1933, PRMT7

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Tested Applications

• Positive WB detected in: HeLa cells, HSC-T6 cells, HepG2 cells, Jurkat cells
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

67669-1-Ig targets PRMT7 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PRMT7 fusion protein Ag30434
• Full Name: protein arginine methyltransferase 7
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 78 kDa
• GenBank Accession Number: BC000146
• Gene Symbol: PRMT7
• Gene ID (NCBI): 54496
• RRID: AB_2882865
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NVM4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein arginine methyltransferases (PRMTs) are responsible for arginine methylation of histone or nonhistone proteins to regulate diverse biological processes, including cell proliferation, differentiation and senescence. PRMT7 is expressed in both embryonic stem and germ cells and is implicated in maintenance of muscle stem cell function. overexpression of PRMT7 has been associated with cancer metastasis. PRMT7 automethylation at residue R531 was reported to enhance breast cancer metastasis.

Publications

Species	Application	Title
human	WB	EMBO J; MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression; Authors - Yan Sun
human	WB	Gastroenterology; Genetic Control of Alternative Splicing and its Distinct Role in Colorectal Cancer Mechanisms; Authors - Ming Zhang