PRMT7 Monoclonal antibody

PRMT7 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

3C5H6

Cat no : 67669-1-Ig

Synonyms

FLJ10640, KIAA1933, PRMT7

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Validation Data Gallery

• 

  WB analysis using 67669-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67669-1-Ig (PRMT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67669-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67669-1-Ig (PRMT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67669-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67669-1-Ig (PRMT7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HSC-T6 cells, HepG2 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below

Product Information

67669-1-Ig targets PRMT7 in WB, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PRMT7 fusion protein Ag30434
• Full Name: protein arginine methyltransferase 7
• Calculated molecular weight: 78 kDa
• Observed molecular weight: 78 kDa
• GenBank accession number: BC000146
• Gene symbol: PRMT7
• Gene ID (NCBI): 54496
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein arginine methyltransferases (PRMTs) are responsible for arginine methylation of histone or nonhistone proteins to regulate diverse biological processes, including cell proliferation, differentiation and senescence. PRMT7 is expressed in both embryonic stem and germ cells and is implicated in maintenance of muscle stem cell function. overexpression of PRMT7 has been associated with cancer metastasis. PRMT7 automethylation at residue R531 was reported to enhance breast cancer metastasis.

Publications

Species	Application	Title
human	WB	Gastroenterology; Genetic Control of Alternative Splicing and its Distinct Role in Colorectal Cancer Mechanisms; Authors - Ming Zhang
human	WB	EMBO J; MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression; Authors - Yan Sun