PRODH Polyclonal antibody

• Featured Product
• KD/KO Validated

PRODH Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 22980-1-AP

Synonyms

HSPOX2, p53 induced gene 6 protein, PIG6, POX, POX2, PRODH, PRODH1, Proline oxidase, Proline oxidase 2, SCZD4, TP53I6

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Validation Data Gallery

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  WB analysis using 22980-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22980-1-AP (PRODH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22980-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22980-1-AP (PRODH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22980-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22980-1-AP (PRODH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cerebellum using 22980-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum slide using 22980-1-AP (PRODH Antibody) at dilution of 1:50(under 10x lens).

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  IHC staining of human cerebellum using 22980-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum slide using 22980-1-AP (PRODH Antibody) at dilution of 1:50(under 40x lens).

• 

  IHC staining of human breast cancer using 22980-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22980-1-AP (PRODH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22980-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22980-1-AP (PRODH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 22980-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22980-1-AP (PRODH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 22980-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22980-1-AP (PRODH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 22980-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22980-1-AP (PRODH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 22980-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22980-1-AP (PRODH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 22980-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22980-1-AP (PRODH Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human skeletal muscle using 22980-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22980-1-AP (PRODH Antibody) at dilution of 1:50(under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, A549 cells, rat liver tissue
• Positive IHC detected in: human cerebellum tissue, human liver cancer tissue, human breast cancer tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

22980-1-AP targets PRODH in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRODH fusion protein Ag18429
• Full Name: proline dehydrogenase (oxidase) 1
• Calculated Molecular Weight: 600 aa, 68 kDa
• Observed Molecular Weight: 56 kDa, 66 kDa
• GenBank Accession Number: BC118597
• Gene Symbol: PRODH
• Gene ID (NCBI): 5625
• RRID: AB_2879191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRODH(Proline dehydrogenase 1, mitochondrial) is also named as PIG6, HSPOX2, PRODH1, PRODH2, POX, SCZD4, TP53I6 and belongs to the proline oxidase family. It is an oxidoreductase involved in the transfer of redox potential across the mitochondrial membrane and catalyzes the rate-limiting oxidation of proline to pyrroline- 5-carboxylate (P5C). High PRODH activity is sufficient to induce mitochondria-mediated apoptosis in the presence of proline. Defects in PRODH are the cause of hyperprolinemia type 1 (HP-1) and defects in PRODH are associated with susceptibility to schizophrenia type 4 (SCZD4). This protein has 3 isoforms produced by alternative splicing with the molecular mass of 68 kDa, 59 kDa and 56 kDa.

Publications

Species	Application	Title
mouse	WB	Nucleic Acids Res; Discordant regulation of eIF2 kinase GCN2 and mTORC1 during nutrient stress.; Authors - Jagannath Misra
rat	WB	Oxid Med Cell Longev; Metabolomic Analysis of the Ameliorative Effect of Enhanced Proline Metabolism on Hypoxia-Induced Injury in Cardiomyocytes.; Authors - Jiacheng Wang ; KD Validated
human	WB	Amino Acids; PINCH-1 promotes Δ1-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.; Authors - Chunhong Cui
human	WB	Amino Acids; Activation of proline metabolism maintains ATP levels during cocaine-induced polyADP-ribosylation.; Authors - Sabyasachi Dash ; KD Validated
	WB,IF	Animals (Basel); Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine.; Authors - Chengwen Feng
human	WB	Nat Commun; Kindlin-2 links mechano-environment to proline synthesis and tumor growth.; Authors - Ling Guo