PRPF31 Fusion Protein
e coli.-derived, PET28a
Cat no : Ag26796
DKFZp566J153; NY-BR-99; PRP31; RP11
(101-212 aa encoded by NM_015629)
|Please contact the lab for more information.
|85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
|The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
Reconstitution and Storage
Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
After reconstitution with sterile water, if glycerol has no effect on subsequent experiments, it is recommended to add an equal volume of glycerol for long-term storage (see Stability and Storage for more details).
If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
|The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature (see below).
|Stability and Storage
|Store for up to 12 months at -20°C to -80°C as lyophilized powder.
|Storage of Reconstituted Protein
Short-term storage: Store at 2-8°C for (1-2 weeks).
Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.