PRPF6 Polyclonal antibody, PBS Only

• 

  WB analysis of HEK-293 using 23929-1-AP (same clone as 23929-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 23929-1-AP (same clone as 23929-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 23929-1-AP (same clone as 23929-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 23929-1-AP (same clone as 23929-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 23929-1-AP (same clone as 23929-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 23929-1-AP (same clone as 23929-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 23929-1-AP (same clone as 23929-1-PBS)

  IP result of anti-PRPF6 (IP:23929-1-AP, 4ug; Detection:23929-1-AP 1:300) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 23929-1-AP (same clone as 23929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23929-1-AP (PRPF6 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 23929-1-AP (same clone as 23929-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23929-1-AP (PRPF6 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 23929-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PRPF6 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 23929-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

---

Product Information

23929-1-PBS targets PRPF6 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13817

  Product name: Recombinant human PRPF6 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 592-941 aa of BC001666

  Sequence: SLEALLQRAVAHCPKAEVLWLMGAKSKWLAGDVPAARSILALAFQANPNSEEIWLAAVKLESENDEYERARRLLAKARSSAPTARVFMKSVKLEWVQDNIRAAQDLCEEALRHYEDFPKLWMMKGQIEEQKEMMEKAREAYNQGLKKCPHSTPLWLLLSRLEEKIGQLTRARAILEKSRLKNPKNPGLWLESVRLEYRAGLKNIANTLMAKALQECPNSGILWSEAIFLEARPQRRTKSVDALKKCEHDPHVLLAVAKLFWSQRKITKAREWFHRTVKIDSDLGDAWAFFYKFELQHGTEEQQEEVRKRCESAEPRHGELWCAVSKDIANWQKKIGDILRLVAGRIKNTF

• Full Name: PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae)
• Calculated Molecular Weight: 941 aa, 107 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC001666
• Gene Symbol: PRPF6
• Gene ID (NCBI): 24148
• RRID: AB_2879365
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: O94906
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PRPF6 is involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, it can enhance dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation.