PRPF6 Polyclonal antibody

• Featured Product
• KD/KO Validated

PRPF6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 23929-1-AP

Synonyms

ANT 1, C20orf14, hPrp6, Pre mRNA processing factor 6, PRP6 homolog, PRPF6, TOM, U5 102 kDa protein, U5 102K

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Validation Data Gallery

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  WB analysis of HEK-293 using 23929-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 23929-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23929-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 23929-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 23929-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 23929-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23929-1-AP (PRPF6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 23929-1-AP

  IP result of anti-PRPF6 (IP:23929-1-AP, 4ug; Detection:23929-1-AP 1:300) with HeLa cells lysate 3200ug.

• 

  IHC staining of human testis using 23929-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23929-1-AP (PRPF6 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 23929-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23929-1-AP (PRPF6 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

23929-1-AP targets PRPF6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRPF6 fusion protein Ag13817
• Full Name: PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae)
• Calculated Molecular Weight: 941 aa, 107 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC001666
• Gene Symbol: PRPF6
• Gene ID (NCBI): 24148
• RRID: AB_2879365
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRPF6 is involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, it can enhance dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation.

Publications

Species	Application	Title
human	WB	Hum Genet; A CRISPR and high-content imaging assay compliant with ACMG/AMP guidelines for clinical variant interpretation in ciliopathies.; Authors - Liliya Nazlamova
mouse	WB,IF,IHC	Cancer Sci; Splicing factor PRPF6 upregulates oncogenic androgen receptor signaling pathway in hepatocellular carcinoma.; Authors - Huijuan Song ; KD Validated
human	WB	Int J Biol Sci; PRPF6 promotes androgen receptor/androgen receptor-variant 7 actions in castration-resistant prostate cancer cells.; Authors - Wei Liu ; KD Validated
human,mouse	IF,WB	Front Genet; Microtubule modification defects underlie cilium degeneration in cell models of retinitis pigmentosa associated with pre-mRNA splicing factor mutations; Authors - Liliya Nazlamova
human	WB	Cell Rep; SART3 reads methylarginine-marked glycine- and arginine-rich motifs; Authors - Yalong Wang