PRPF8 Polyclonal antibody, PBS Only

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  WB analysis of Jurkat using 11171-1-AP (same clone as 11171-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11171-1-AP (same clone as 11171-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 11171-1-AP (same clone as 11171-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 11171-1-AP (same clone as 11171-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11171-1-AP (same clone as 11171-1-PBS)

  IP result of anti-PRPF8 (IP:11171-1-AP, 4ug; Detection:11171-1-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 11171-1-AP (same clone as 11171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11171-1-AP (same clone as 11171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 11171-1-AP (same clone as 11171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11171-1-PBS in a different storage buffer formulation.

PRPF8 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 11171-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

11171-1-PBS targets PRPF8 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1652

  Product name: Recombinant human PRPF8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 2037-2335 aa of BC064370

  Sequence: SQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

• Full Name: PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae)
• Calculated Molecular Weight: 274 kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: BC064370
• Gene Symbol: PRPF8
• Gene ID (NCBI): 10594
• RRID: AB_2171179
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6P2Q9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PRPF8, a 220kd protein,is a component of mammalian spliceosomes, large multiprotein complexes involved in the removal of introns from mRNA precursor. It has a central structural role in the architecture of the U5 small nuclear ribonucleoprotein (snRNP) particle.