PRPF8 Polyclonal antibody

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• PRPF8 Polyclonal antibody (11171-1-AP)

PRPF8 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 11171-1-AP Copied!

Publications (16)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, CoIP, RIP, ELISA

Synonyms

220 kDa U5 snRNP-specific protein, HPRP8, p220, Pre-mRNA-processing-splicing factor 8, PRP8

• 

  WB analysis of Jurkat using 11171-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 11171-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human placenta using 11171-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 11171-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 11171-1-AP

  IP result of anti-PRPF8 (IP:11171-1-AP, 4ug; Detection:11171-1-AP 1:500) with HeLa cells lysate 2000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 11171-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 11171-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 11171-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, HeLa cells, human placenta tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11171-1-AP targets PRPF8 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1652

  Product name: Recombinant human PRPF8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 2037-2335 aa of BC064370

  Sequence: SQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

• Full Name: PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae)
• Calculated Molecular Weight: 274 kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: BC064370
• Gene Symbol: PRPF8
• Gene ID (NCBI): 10594
• RRID: AB_2171179
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6P2Q9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRPF8, a 220kd protein,is a component of mammalian spliceosomes, large multiprotein complexes involved in the removal of introns from mRNA precursor. It has a central structural role in the architecture of the U5 small nuclear ribonucleoprotein (snRNP) particle.

Publications

What published studies show

The PRPF8 polyclonal antibody (Cat# 11171-1-AP) has 16 citations across 13 journals including Molecular Cell, Neuron, Gastroenterology, Nucleic Acids Research, Signal Transduction and Targeted Therapy, Genes & Development, Cell Reports, PLoS Biology, Communications Biology, Cell Oncology, RNA, JBC, and PLoS ONE. Research fields span spliceosome mechanics, alternative splicing regulation, cancer biology (hepatocellular, colorectal, pancreatic, and bladder cancers), neurodevelopment, and mRNA nuclear export.

Automatically generated from the 16 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Signal Transduct Target Ther; KAT2A-driven succinylation of SRSF11 enforces spliceosome-mediated RAD52 splicing to promote homologous recombination and radioresistance in hepatocellular carcinoma; Authors - Jun Wu
human	RIP	Gastroenterology; Genetic Control of Alternative Splicing and its Distinct Role in Colorectal Cancer Mechanisms; Authors - Ming Zhang
mouse,human	WB	Neuron; Species-specific FMRP regulation of RACK1 is critical for prenatal cortical development; Authors - Minjie Shen
human	WB	Mol Cell; Productive mRNA chromatin escape is promoted by PRMT5 activity; Authors - Joseph D DeAngelo
human	WB	Mol Cell; Mechanism of exon ligation by human spliceosome.; Authors - Xiechao Zhan
human	WB	Mol Cell; ZC3H14 facilitates backsplicing by binding to exon-intron boundary and 3' UTR; Authors - Qiqi Li

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

One reviewer rated this antibody 5/5 stars for Western Blot on HeLa cells at a 1:1000 dilution (overnight at 4°C), reporting a strong, clean band at the expected molecular weight. Overall, the feedback is positive, confirming reliable performance in WB applications.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Roy (Verified Customer) (10-05-2025)

Great band at the correct size. Antibody used overnight at 4°C - 1/1000 dilution (Western-Blot).

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa