Tested Applications
| Positive WB detected in | mouse eye tissue, rat eye tissue |
| Positive IF-P detected in | mouse eye tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18109-1-AP targets PRPH2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12555 Product name: Recombinant human PRPH2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 124-346 aa of BC074720 Sequence: GSLENTLGQGLKNGMKYYRDTDTPGRCFMKKTIDMLQIEFKCCGNNGFRDWFEIQWISNRYLDFSSKEVKDRIKSNVDGRYLVDGVPFSCCNPSSPRPCIQYQITNNSAHYSYDHQTEELNLWVRGCRAALLSYYSSLMNSMGVVTLLIWLFEVTITIGLRYLQTSLDGVSNPEESESESEGWLLEKSVPETWKAFLESVKKLGKGNQVEAEGAGAGQAPEAG Predict reactive species |
| Full Name | peripherin 2 (retinal degeneration, slow) |
| Calculated Molecular Weight | 346 aa, 39 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC074720 |
| Gene Symbol | PRPH2 |
| Gene ID (NCBI) | 5961 |
| RRID | AB_10665364 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P23942 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Peripherin-2 (PRPH2), also known as retinal degeneration slow protein (RDS), is a photoreceptor-specific tetraspanin protein implicated in outer segment disk morphogenesis. It may function as an adhesion molecule involved in stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. Mutations in peripherin-2 are responsible for various retinal degenerative diseases including autosomal dominant retinitis pigmentosa (ADRP).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PRPH2 antibody 18109-1-AP | Download protocol |
| WB protocol for PRPH2 antibody 18109-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PRPH2 Polyclonal antibody (Cat# 18109-1-AP) has 27 citations spanning 19 journals, including PNAS, Molecular Therapy, FASEB Journal, Journal of Biological Chemistry, iScience, and Cell Death & Disease. Research fields covered include retinal degeneration, photoreceptor biology, gene therapy, retinal transplantation, ciliopathies (Bardet-Biedl syndrome), retinitis pigmentosa, central areolar choroidal dystrophy, and synaptic remodeling. Applications cited include WB, IF, IHC, and IP across human, mouse, rat, and zebrafish models.
| Species | Application | Title |
|---|---|---|
Biomaterials Hydrogel-mediated co-transplantation of retinal pigmented epithelium and photoreceptors restores vision in an animal model of advanced retinal degeneration. | ||
Cell Death Dis Prph2 knock-in mice recapitulate human central areolar choroidal dystrophy retinal degeneration and exhibit aberrant synaptic remodeling and microglial activation | ||
Mol Ther Exogenous photoreceptor-specific N-glycosylated PROM1 rescues retinal degeneration in patient and mouse models. | ||
Proc Natl Acad Sci U S A Reversal of end-stage retinal degeneration and restoration of visual function by photoreceptor transplantation. | ||
Proc Natl Acad Sci U S A Transplantation of human embryonic stem cell-derived retinal tissue in two primate models of retinal degeneration. | ||
Proc Natl Acad Sci U S A Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. |
Reviews
What customers say
Both reviewers gave this antibody a 5-star rating for Western Blot. One user reported excellent results at a 1:2000 dilution, while the other found it performed very well at 1 µg/ml in whole retina lysate. Overall, customers are highly satisfied with its WB performance, noting clear and reliable results across different dilutions and sample types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
XINYU (Verified Customer) (10-09-2025) | Nice results for western blot application!
|
Melina (Verified Customer) (05-13-2024) | Worked very well in western blots of whole retina lysate
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