PSG1 Monoclonal antibody

PSG1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1C6C4

Cat no : 67269-1-Ig

Synonyms

Fetal liver non-specific cross-reactive antigen 1/2, DHFRP2, CD66f, CD66 antigen-like family member F, B1G1

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Validation Data Gallery

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  WB analysis of T-47D using 67269-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 67269-1-Ig (PSG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 67269-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67269-1-Ig (PSG1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 67269-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67269-1-Ig (PSG1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human placenta using 67269-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using PSG1 antibody (67269-1-Ig, Clone: 1C6C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human placenta using 67269-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using PSG1 antibody (67269-1-Ig, Clone: 1C6C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: T-47D cells, L02 cells, NCCIT cells, JAR cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67269-1-Ig targets PSG1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSG1 fusion protein Ag28604
• Full Name: pregnancy specific beta-1-glycoprotein 1
• Calculated Molecular Weight: 426 aa, 48 kDa
• Observed Molecular Weight: 54-70 kDa
• GenBank Accession Number: BC022338
• Gene Symbol: PSG1
• Gene ID (NCBI): 5669
• RRID: AB_2882539
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.