Tested Applications
| Positive WB detected in | Jurkat cells, human kidney tissue |
| Positive IP detected in | Raji cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14859-1-AP targets PSMB8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6660 Product name: Recombinant human PSMB8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-272 aa of BC001114 Sequence: MLIGTPTPRDTTPSSWLTSSLLVEAAPLDDTTLPTPVSSGCPGLEPTEFFQSLGGDGERNVQIEMAHGTTTLAFKFQHGVIAAVDSRASAGSYISALRVNKVIEINPYLLGTMSGCAADCQYWERLLAKECRLYYLRNGERISVSAASKLLSNMMCQYRGMGLSMGSMICGWDKKGPGLYYVDEHGTRLSGNMFSTGSGNTYAYGVMDSGYRPNLSPEEAYDLGRRAIAYATHRDSYSGGVVNMYHMKEDGWVKVESTDVSDLLHQYREANQ Predict reactive species |
| Full Name | proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7) |
| Calculated Molecular Weight | 30 kDa |
| Observed Molecular Weight | 23 kDa |
| GenBank Accession Number | BC001114 |
| Gene Symbol | PSMB8 |
| Gene ID (NCBI) | 5696 |
| RRID | AB_2268923 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P28062 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PSMB8(Proteasome subunit beta type-8) is also named as LMP7, PSMB5i, RING10, Y2 and belongs to the peptidase T1B family. The gene encodes the chymotrypsin-like catalytic subunit of the immunoproteasome(PMID: 19525961). PSMB8 has a role in controlling pathogenic immune responses and may be a target in autoimmune disorders. Its prosequence is not essential for incorporation of PSMB8 into the maturing proteasome, but it increased the efficiency of PSMB8 incorporation and proteasome maturation(PMID: 10926487). The pro-PSMB8 is a 276aa protein with the molecular mass of 30 kDa, and the mature form is about 23kDa due to the 72aa propeptide cleaved. Defects in PSMB8 are the cause of Nakajo syndrome (NKJO).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PSMB8 antibody 14859-1-AP | Download protocol |
| IP protocol for PSMB8 antibody 14859-1-AP | Download protocol |
| WB protocol for PSMB8 antibody 14859-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-PSMB8 (LMP7) antibody (Cat# 14859-1-AP) has 21 citations across journals including Cell, Cell Reports, Journal of Neuroinflammation, Journal of Translational Medicine, Frontiers in Immunology, JCI Insight, Science Reports, International Journal of Cancer, and American Journal of Pathology. Research fields span neuroimmunology (multiple sclerosis, EAE), oncology (bladder, pancreatic, thyroid, breast cancer), inflammatory diseases (ulcerative colitis, airway inflammation), proteasome biology, T cell immunology, nephrology, and dermatology.
| Species | Application | Title |
|---|---|---|
Cell EBV infection and HLA-DR15 jointly drive multiple sclerosis by myelin peptide presentation. | ||
J Neuroinflammation β-arrestin1 regulates astrocytic reactivity via Drp1-dependent mitochondrial fission: implications in postoperative delirium | ||
J Transl Med Immunoproteasome subunits are novel signatures for predicting efficacy of immunotherapy in muscle invasive bladder cancer | ||
ACS Appl Mater Interfaces An Astrocyte-Targeted Nanoplatform with Ultrasound-Responsive Fluorescence Switch for Synergistic Chemo-Sonothermal Therapy of Cognitive Impairment. | ||
Cell Rep Neuronal hyperactivity becomes mTORC1 independent due to transcriptional changes in tuberous sclerosis complex disease models. | ||
Cell Rep ECM29/proteasome-mediated self-antigen generation by CNS-resident neuroglia promotes regulatory T cell activation |
