PSMC3IP Polyclonal antibody

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  WB analysis using 11339-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11339-1-AP (HOP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 11339-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11339-1-AP (HOP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 11339-1-AP

  IP result of anti-HOP2 (IP:11339-1-AP, 4ug; Detection:11339-1-AP 1:500) with Jurkat cells lysate 3200ug.

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  IHC staining of mouse testis using 11339-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11339-1-AP (HOP2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 11339-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11339-1-AP (HOP2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 11339-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11339-1-AP (HOP2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 11339-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11339-1-AP (HOP2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11339-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HOP2 antibody (11339-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

PSMC3IP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11339-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PSMC3-interacting protein, PSMC3 interacting protein, Proteasome 26S ATPase subunit 3-interacting protein, HOP2, Homologous-pairing protein 2 homolog

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Tested Applications

• Positive WB detected in: HEK-293 cells, K-562 cells, Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: mouse testis tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Product Information

11339-1-AP targets PSMC3IP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMC3IP fusion protein Ag1883
• Full Name: PSMC3 interacting protein
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 24-29 kDa
• GenBank Accession Number: BC008792
• Gene Symbol: PSMC3IP
• Gene ID (NCBI): 29893
• RRID: AB_2172642
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P2W1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMC3IP, also called HOP2, encodes a protein that functions in meiotic recombination. It is a subunit of the PSMC3IP/MND1 complex, which interacts with PSMC3/TBP1 to stimulate DMC1- and RAD51-mediated strand exchange during meiosis. The protein encoded by this gene can also co-activate ligand-driven transcription mediated by estrogen, androgen, glucocorticoid, progesterone, and thyroid nuclear receptors. Mutations in this gene cause XX female gonadal dysgenesis.

Publications

Species	Application	Title
human	WB	Cell; Interchromosomal Homology Searches Drive Directional ALT Telomere Movement and Synapsis.; Authors - Nam Woo Cho ; KD Validated
mouse	IF	Nucleic Acids Res; RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis; Authors - Chao Liu
human	WB	Cell Rep; MND1 and PSMC3IP control PARP inhibitor sensitivity in mitotic cells; Authors - Anabel Zelceski
human		Nat Commun; Promotion of homology-directed DNA repair by polyamines.; Authors - Chih-Ying Lee
human	WB,IF	Oncotarget; A study of meiomitosis and novel pathways of genomic instability in cutaneous T-cell lymphomas (CTCL).; Authors - Matthew Tsang