|Positive WB detected in||Jurkat cells, K-562 cells, rat spleen tissue, HeLa cells, Raji cells, NIH/3T3 cells, RAW 264.7 cells|
|Positive IHC detected in||human lung cancer tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14899-1-AP targets PSMD4 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PSMD4 fusion protein Ag6691|
|Full Name||proteasome (prosome, macropain) 26S subunit, non-ATPase, 4|
|Calculated molecular weight||41 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC002365|
|Gene ID (NCBI)||5710|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PSMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. PSMD4 is a 50-kD subunit of the regulatory complex of the 26S proteasome. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.
|Product Specific Protocols|
|WB protocol for PSMD4 antibody 14899-1-AP||Download protocol|
|IHC protocol for PSMD4 antibody 14899-1-AP||Download protocol|
|IF protocol for PSMD4 antibody 14899-1-AP||Download protocol|
|FC protocol for PSMD4 antibody 14899-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Interaction of HPV E6 oncoproteins with specific proteasomal subunits.
Biochim Biophys Acta Mol Basis Dis
Defective proteasome biogenesis into skin fibroblasts isolated from Rett syndrome subjects with MeCP2 non-sense mutations.
J Biol Chem
Chaperone-mediated 26S Proteasome Remodeling Facilitates Free K63 Ubiquitin Chain Production and Aggresome Clearance.
Mol Cell Proteomics
Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7.
J Cell Sci
S5a binds to death receptor-6 to induce THP-1 monocytes to differentiate through the activation of the NF-κB pathway.
The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.