Validation Data Gallery
|Positive WB detected in||Jurkat cells, K-562 cells, rat spleen tissue, HeLa cells, Raji cells, NIH/3T3 cells, RAW 264.7 cells|
|Positive IHC detected in||human lung cancer tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14899-1-AP targets PSMD4 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PSMD4 fusion protein Ag6691|
|Full Name||proteasome (prosome, macropain) 26S subunit, non-ATPase, 4|
|Calculated molecular weight||41 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC002365|
|Gene ID (NCBI)||5710|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PSMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. PSMD4 is a 50-kD subunit of the regulatory complex of the 26S proteasome. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.
|Product Specific Protocols|
|WB protocol for PSMD4 antibody 14899-1-AP||Download protocol|
|IHC protocol for PSMD4 antibody 14899-1-AP||Download protocol|
|IF protocol for PSMD4 antibody 14899-1-AP||Download protocol|
|FC protocol for PSMD4 antibody 14899-1-AP||Download protocol|
|Click here to view our Standard Protocols|
BMC Dev Biol
Generation and identification of a conditional knockout allele for the PSMD11 gene in mice.
Ubiquitin receptors play redundant roles in the proteasomal degradation of the p53 repressor MDM2.
Interaction of HPV E6 oncoproteins with specific proteasomal subunits.
Proteasome inhibition by bortezomib parallels a reduction in head and neck cancer cells growth, and an increase in tumor-infiltrating immune cells.
Biochim Biophys Acta Mol Basis Dis
Defective proteasome biogenesis into skin fibroblasts isolated from Rett syndrome subjects with MeCP2 non-sense mutations.
J Biol Chem
Chaperone-mediated 26S Proteasome Remodeling Facilitates Free K63 Ubiquitin Chain Production and Aggresome Clearance.