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PSMD4 Polyclonal antibody

PSMD4 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14899-1-AP

Synonyms

AF, AF 1, Antisecretory factor 1, ASF, MCB1, PSMD4, pUB R5, Rpn10, S5A



Tested Applications

Positive WB detected inJurkat cells, K-562 cells, rat spleen tissue, HeLa cells, Raji cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman lung cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14899-1-AP targets PSMD4 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMD4 fusion protein Ag6691
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
Calculated molecular weight 41 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC002365
Gene symbol PSMD4
Gene ID (NCBI) 5710
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. PSMD4 is a 50-kD subunit of the regulatory complex of the 26S proteasome. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.

Protocols

Product Specific Protocols
WB protocol for PSMD4 antibody 14899-1-APDownload protocol
IHC protocol for PSMD4 antibody 14899-1-APDownload protocol
IF protocol for PSMD4 antibody 14899-1-APDownload protocol
FC protocol for PSMD4 antibody 14899-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Nat Neurosci

Loss of nuclear UBE3A causes electrophysiological and behavioral deficits in mice and is associated with Angelman syndrome.

Authors - Rossella Avagliano Trezza
mouseIF

J Cell Biol

The transition zone protein Rpgrip1l regulates proteasomal activity at the primary cilium.

Authors - Christoph Gerhardt
humanWB

Cancer Res

The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.

Authors - Xiuxia Liu
humanWB

Oncogene

The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.

Authors - Sparks A A
humanWB

J Cell Sci

S5a binds to death receptor-6 to induce THP-1 monocytes to differentiate through the activation of the NF-κB pathway.

Authors - Zheng Wang
humanWB

Mol Cell Proteomics

Proteaphagy in Mammalian Cells Can Function Independent of ATG5/ATG7.

Authors - Tatjana Goebel