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  • KD/KO Validated

PSMD4 Polyclonal antibody

PSMD4 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14899-1-AP


AF, AF 1, Antisecretory factor 1, ASF, MCB1, PSMD4, pUB R5, Rpn10, S5A

Tested Applications

Positive WB detected inJurkat cells, K-562 cells, rat spleen tissue, HeLa cells, Raji cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman lung cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14899-1-AP targets PSMD4 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMD4 fusion protein Ag6691
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
Calculated molecular weight 41 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC002365
Gene symbol PSMD4
Gene ID (NCBI) 5710
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. PSMD4 is a 50-kD subunit of the regulatory complex of the 26S proteasome. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.


Product Specific Protocols
WB protocol for PSMD4 antibody 14899-1-APDownload protocol
IHC protocol for PSMD4 antibody 14899-1-APDownload protocol
IF protocol for PSMD4 antibody 14899-1-APDownload protocol
FC protocol for PSMD4 antibody 14899-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



BMC Dev Biol

Generation and identification of a conditional knockout allele for the PSMD11 gene in mice.

Authors - Linlin Zhao


Ubiquitin receptors play redundant roles in the proteasomal degradation of the p53 repressor MDM2.

Authors - Alison Sparks
  • KD Validated


Interaction of HPV E6 oncoproteins with specific proteasomal subunits.

Authors - Tomai? Vjekoslav V

Sci Rep

Proteasome inhibition by bortezomib parallels a reduction in head and neck cancer cells growth, and an increase in tumor-infiltrating immune cells.

Authors - Monica Benvenuto

Biochim Biophys Acta Mol Basis Dis

Defective proteasome biogenesis into skin fibroblasts isolated from Rett syndrome subjects with MeCP2 non-sense mutations.

Authors - Diego Sbardella

J Biol Chem

Chaperone-mediated 26S Proteasome Remodeling Facilitates Free K63 Ubiquitin Chain Production and Aggresome Clearance.

Authors - Priyaanka Nanduri
  • KD Validated