PSME2 Polyclonal antibody, PBS Only

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  WB analysis of MCF-7 using 12937-2-AP (same clone as 12937-2-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12937-2-AP (PSME2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 12937-2-AP (same clone as 12937-2-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12937-2-AP (PSME2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12937-2-AP (same clone as 12937-2-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12937-2-AP (PSME2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

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  WB analysis of mouse pancreas using 12937-2-AP (same clone as 12937-2-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12937-2-AP (PSME2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 12937-2-AP (same clone as 12937-2-PBS)

  IP result of anti-PSME2 (IP:12937-2-AP, 4ug; Detection:12937-2-AP 1:500) with MCF-7 cells lysate 2000ug. This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12937-2-AP (same clone as 12937-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12937-2-AP (PSME2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12937-2-AP (same clone as 12937-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12937-2-AP (PSME2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 12937-2-AP (same clone as 12937-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PSME2 antibody (12937-2-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 12937-2-PBS in a different storage buffer formulation.

PSME2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 12937-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

REG-beta, REGbeta, REG beta, PA28beta, PA28B

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Product Information

12937-2-PBS targets PSME2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3986

  Product name: Recombinant human PSME2 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 1-239 aa of BC019885

  Sequence: MAKPCGVRLSGEARKQVEVFRQNLFQEAEEFLYRFLPQKIIYLNQLLQEDSLNVADLTSLRAPLDIPIPDPPPKDDEMETDKQEKKEVPKCGFLPGNEKVLSLLALVKPEVWTLKEKCILVITWIQHLIPKIEDGNDFGVAIQEKVLERVNAVKTKVEAFQTTISKYFSERGDAVAKASKETHVMDYRALVHERDEAAYGELRAMVLDLRAFYAELYHIISSNLEKIVNPKGEEKPSMY

• Full Name: proteasome (prosome, macropain) activator subunit 2 (PA28 beta)
• Calculated Molecular Weight: 239 aa, 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC019885
• Gene Symbol: PSME2
• Gene ID (NCBI): 5721
• RRID: AB_2300265
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UL46
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.