PSPC1 Polyclonal antibody

• 

  WB analysis using 16714-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16714-1-AP (PSPC1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16714-1-AP

  WB result of PSPC1 antibody (16714-1-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSPC1 transfected HEK-293 cells.

• 

  IP experiment of HEK-293 using 16714-1-AP

  IP result of anti-PSPC1 (IP:16714-1-AP, 4ug; Detection:16714-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

• 

  IHC staining of human placenta using 16714-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16714-1-AP (PSPC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 16714-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16714-1-AP (PSPC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 16714-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 16714-1-AP (PSPC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

PSPC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16714-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

PSP1, PSPC 1

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, PC-3 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 3 publications below
• RIP: See 1 publications below

Product Information

16714-1-AP targets PSPC1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10136

  Product name: Recombinant human PSPC1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-351 aa of BC014184

  Sequence: MMLRGNLKQVRIEKNPARLRALESAVGESEPAAAAAMALALAGEPAPPAPAPPEDHPDEEMGFTIDIKSFLKPGEKTYTQRCRLFVGNLPTDITEEDFKRLFERYGEPSEVFINRDRGFGFIRLESRTLAEIAKAELDGTILKSRPLRIRFATHGAALTVKNLSPVVSNELLEQAFSQFGPVEKAVVVVDDRGRATGKGFVEFAAKPPARKALERCGDGAFLLTTTPRPVIVEPMEQFDDEDGLPEKLMQKTQQYHKEREQPPRFAQPGTFEFEYASRWKALDEMEKQQREQVDRNIREAKEKLEAEMEAARHEHQLMLMRQDLMRRQEELRRLEELRNQELQKRKQIQLR

• Full Name: paraspeckle component 1
• Calculated Molecular Weight: 523 aa, 59 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC014184
• Gene Symbol: PSPC1
• Gene ID (NCBI): 55269
• RRID: AB_2878302
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WXF1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSPC1 (paraspeckle component 1), a known paraspeckle biomarker, is a putative transcription factor that belongs to the Drosophila behavior/human splicing (DBHS) family. PSPC1 was recently identified as a contextual determinant of tumor progression in multiple cancer types involving oncogenic reprogramming to switch proapoptotic TGF-β to prometastatic TGF-β via hijacking of Smad2/3 targeting. Upregulated PSPC1 is correlated with advanced tumor stages and poor survival of patients with breast, lung, and liver cancers. Upregulated PSPC1 potentiates expression of mesenchymal markers, EMT transcription factors (EMT-TF), cancer stem-like cell transcription factors (CSC-TF), and c-Myc-related proliferation genes, thus promoting migration, invasion, spheroid formation, tumor formation, and metastasis.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Drug Resist Updat; Cell membrane-camouflaged bufalin targets NOD2 and overcomes multidrug resistance in pancreatic cancer; Authors - Wei Zhang
human	WB	Nat Commun; K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis; Authors - Xiao-Lan Zhang
human	WB	Nucleic Acids Res; mRNAs are sorted for export or degradation before passing through nuclear speckles.; Authors - Jing Fan
human	WB,IF	Cancer Lett; SKP2 promotes the metastasis of pancreatic ductal adenocarcinoma by suppressing TRIM21-mediated PSPC1 degradation; Authors - Jiahui Yuan
mouse	WB	Nat Commun; Capture of RNA-binding proteins across mouse tissues using HARD-AP; Authors - Yijia Ren
human	IF	Nat Commun; Virus-modified paraspeckle-like condensates are hubs for viral RNA processing and their formation drives genomic instability; Authors - Katherine L Harper