PTBP2 Polyclonal antibody, PBS Only

• 

  WB analysis of mouse heart using 55186-1-AP (same clone as 55186-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 55186-1-AP (same clone as 55186-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 55186-1-AP (same clone as 55186-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 55186-1-AP (same clone as 55186-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 55186-1-AP (same clone as 55186-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 55186-1-AP (same clone as 55186-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 55186-1-AP (same clone as 55186-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 55186-1-AP (same clone as 55186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55186-1-AP (PTBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 55186-1-AP (same clone as 55186-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55186-1-AP (PTBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 55186-1-AP (same clone as 55186-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 55186-1-AP (PTBP2 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 55186-1-AP (same clone as 55186-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 55186-1-AP (PTBP2 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55186-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PTBP2 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 55186-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

PTB like protein, PTB, Polypyrimidine tract-binding protein 2, Neurally-enriched homolog of PTB, Neural polypyrimidine tract-binding protein

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Product Information

55186-1-PBS targets PTBP2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: polypyrimidine tract binding protein 2
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57-60 kDa
• GenBank Accession Number: NM_021190
• Gene Symbol: PTBP2
• Gene ID (NCBI): 58155
• RRID: AB_10837230
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UKA9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PTBP2, also named as NPTB, PTB and PTBLP, is a RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. It may antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. Beside its function in pre-mRNA splicing, PTBP2 plays also a role in the regulation of translation. Isoform 5 has a reduced affinity for RNA. (PMID:11003644) . Protein may exist in a dimeric form with a MW of 110-120 kDa (PMID: 20064465). This antibody is specific to PTBP2.