PTBP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PTBP2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 55186-1-AP

Synonyms

brPTB, PTB, PTB like protein, PTBLP, PTBP2

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Validation Data Gallery

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  WB analysis of mouse heart using 55186-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 55186-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 55186-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 55186-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 55186-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55186-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 55186-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 55186-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55186-1-AP (PTBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 55186-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55186-1-AP (PTBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 55186-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 55186-1-AP (PTBP2 antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 55186-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 55186-1-AP (PTBP2 antibody), at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse brain tissue, mouse skeletal muscle tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

55186-1-AP targets PTBP2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: polypyrimidine tract binding protein 2
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57-60 kDa
• GenBank Accession Number: NM_021190
• Gene Symbol: PTBP2
• Gene ID (NCBI): 58155
• RRID: AB_10837230
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTBP2, also named as NPTB, PTB and PTBLP, is a RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. It may antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. Beside its function in pre-mRNA splicing, PTBP2 plays also a role in the regulation of translation. Isoform 5 has a reduced affinity for RNA. (PMID:11003644) . Protein may exist in a dimeric form with a MW of 110-120 kDa (PMID: 20064465). This antibody is specific to PTBP2.

Publications

Species	Application	Title
human	WB	Cell Rep; PTBP1 and PTBP2 Repress Nonconserved Cryptic Exons.; Authors - Jonathan P Ling ; KD Validated
human,mouse	WB	Mol Cell Biol; RBM4 modulates radial migration via alternative splicing of Dab1 during cortex development.; Authors - Dhananjaya D
mouse	WB,IF,IP	Nat Commun; Cytosolic Ptbp2 modulates axon growth in motoneurons through axonal localization and translation of Hnrnpr; Authors - Saeede Salehi ; KD Validated
human	WB	Nucleic Acids Res; N-terminal domain of polypyrimidine-tract binding protein is a dynamic folding platform for adaptive RNA recognition; Authors - Fred F Damberger