Tested Applications
| Positive WB detected in | mouse heart tissue, mouse brain tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55186-1-AP targets PTBP2 in WB, IHC, IF-P, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | polypyrimidine tract binding protein 2 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 57-60 kDa |
| GenBank Accession Number | NM_021190 |
| Gene Symbol | PTBP2 |
| Gene ID (NCBI) | 58155 |
| RRID | AB_10837230 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UKA9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PTBP2, also named as NPTB, PTB and PTBLP, is a RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. It may antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. Beside its function in pre-mRNA splicing, PTBP2 plays also a role in the regulation of translation. Isoform 5 has a reduced affinity for RNA. (PMID:11003644) . Protein may exist in a dimeric form with a MW of 110-120 kDa (PMID: 20064465). This antibody is specific to PTBP2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PTBP2 antibody 55186-1-AP | Download protocol |
| IHC protocol for PTBP2 antibody 55186-1-AP | Download protocol |
| WB protocol for PTBP2 antibody 55186-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PTBP2 antibody (Cat# 55186-1-AP) has 10 total citations published in journals including Nature Communications, Nucleic Acids Research, EMBO Journal, Cell Reports, Journal of Neurochemistry, Frontiers in Molecular Neuroscience, Discovery Oncology, Molecular Cell Biology, and RNA. Research fields span RNA splicing regulation, axon growth and motoneuron biology, dysmyelination, hepatocellular carcinoma, cortical development, male fertility/meiosis, and U7 snRNA processing.
| Species | Application | Title |
|---|---|---|
Nat Commun Cytosolic Ptbp2 modulates axon growth in motoneurons through axonal localization and translation of Hnrnpr
| ||
Nucleic Acids Res N-terminal domain of polypyrimidine-tract binding protein is a dynamic folding platform for adaptive RNA recognition | ||
EMBO J An ultra-conserved poison exon in the Tra2b gene encoding a splicing activator is essential for male fertility and meiotic cell division | ||
Cell Rep Interrogation of RNA-bound proteome with XRNAX illuminates molecular alterations in the mouse brain affected with dysmyelination | ||
J Neurochem Isoform-Specific Splicing of ANK2 by PTBP2 Orchestrates Retinal Pigment Epithelial-to-Neuron Fate Conversion. |
Reviews
What customers say
One reviewer (Xin Chen) rated this antibody 5/5 stars, using it at a 1:2000 dilution for Western Blot on mouse brain lysate. They confirmed it detects endogenous PTBP2 at approximately 55 kD, consistent with the expected molecular weight. Overall, the review reflects positive performance in detecting the target protein under standard WB conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Xin (Verified Customer) (11-24-2025) | This Ab can detect endo PTBP2 in mouse brain lysate with a MW of about 55kD.
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